中华物理医学与康复杂志
中華物理醫學與康複雜誌
중화물리의학여강복잡지
CHINESE JOURNAL OF PHYSICAL MEDICINE AND REHABILITATION
2010年
5期
333-337
,共5页
刘德山%刘楠%张逸仙%杜厚伟%陈荣华
劉德山%劉楠%張逸仙%杜厚偉%陳榮華
류덕산%류남%장일선%두후위%진영화
脑缺血%康复训练%热休克蛋白70%C-MYC
腦缺血%康複訓練%熱休剋蛋白70%C-MYC
뇌결혈%강복훈련%열휴극단백70%C-MYC
Cerebral ischemia%Rehabilitative training%Heat shock protein 70%C-MYC
目的 探讨跑台运动训练对局灶性脑缺血大鼠神经功能恢复和脑缺血组织中热休克蛋白70(HSP70)及C-MYC表达的影响.方法 将42只清洁级成年雄性SD大鼠分为假手术组6只、模型组18只、运动组18只.模型组、运动组大鼠采用改良的Longs线栓法制备大脑中动脉闭塞(MCAO)脑缺血模型,运动组于造模成功后24 h采用跑台训练器进行运动训练,每周6 d,共2周,其余2组则置于普通笼内饲养,期间可自由活动、进食.采用修正的神经行为学评分方法评价模型组和运动组大鼠MCAO脑缺血后第3,7,14天的神经功能,并断头取脑,采用逆转录-多聚酶链反应(RT-PCR)法、免疫组织化学和Western blot法检测脑缺血组织中HSP70、C-MYC的表达.结果 运动组大鼠脑缺血第7,14天神经功能评分均明显优于模型组(P<0.05或0.01);运动组大鼠脑缺血第7,14天HSF70和C-MYC表达较模型组增强(P<0.05或0.01).结论 运动训练可促进脑缺血大鼠神经功能恢复,其机制可能与上调脑缺血组织中HSP70及C-MYC表达有关.
目的 探討跑檯運動訓練對跼竈性腦缺血大鼠神經功能恢複和腦缺血組織中熱休剋蛋白70(HSP70)及C-MYC錶達的影響.方法 將42隻清潔級成年雄性SD大鼠分為假手術組6隻、模型組18隻、運動組18隻.模型組、運動組大鼠採用改良的Longs線栓法製備大腦中動脈閉塞(MCAO)腦缺血模型,運動組于造模成功後24 h採用跑檯訓練器進行運動訓練,每週6 d,共2週,其餘2組則置于普通籠內飼養,期間可自由活動、進食.採用脩正的神經行為學評分方法評價模型組和運動組大鼠MCAO腦缺血後第3,7,14天的神經功能,併斷頭取腦,採用逆轉錄-多聚酶鏈反應(RT-PCR)法、免疫組織化學和Western blot法檢測腦缺血組織中HSP70、C-MYC的錶達.結果 運動組大鼠腦缺血第7,14天神經功能評分均明顯優于模型組(P<0.05或0.01);運動組大鼠腦缺血第7,14天HSF70和C-MYC錶達較模型組增彊(P<0.05或0.01).結論 運動訓練可促進腦缺血大鼠神經功能恢複,其機製可能與上調腦缺血組織中HSP70及C-MYC錶達有關.
목적 탐토포태운동훈련대국조성뇌결혈대서신경공능회복화뇌결혈조직중열휴극단백70(HSP70)급C-MYC표체적영향.방법 장42지청길급성년웅성SD대서분위가수술조6지、모형조18지、운동조18지.모형조、운동조대서채용개량적Longs선전법제비대뇌중동맥폐새(MCAO)뇌결혈모형,운동조우조모성공후24 h채용포태훈련기진행운동훈련,매주6 d,공2주,기여2조칙치우보통롱내사양,기간가자유활동、진식.채용수정적신경행위학평분방법평개모형조화운동조대서MCAO뇌결혈후제3,7,14천적신경공능,병단두취뇌,채용역전록-다취매련반응(RT-PCR)법、면역조직화학화Western blot법검측뇌결혈조직중HSP70、C-MYC적표체.결과 운동조대서뇌결혈제7,14천신경공능평분균명현우우모형조(P<0.05혹0.01);운동조대서뇌결혈제7,14천HSF70화C-MYC표체교모형조증강(P<0.05혹0.01).결론 운동훈련가촉진뇌결혈대서신경공능회복,기궤제가능여상조뇌결혈조직중HSP70급C-MYC표체유관.
Objective To observe the effects of treadmill training on the recovery of neurological function and the expression of HSP70 and C-MYC in the brains of rats with focal cerebral ischemia. Methods Forty-two male adult Sprague-Dawley rats were randomly divided into a sham group ( n =6), a model group (n =18) and a treadmill exercise group (n=18). Focal cerebral ischemia was induced by right middle cerebral artery occlusion (MCAO) in the model group and exercise group using a modified version of Longa's method. The rats in the treadmill exercise group were given treadmill training 6 d per week for 2 weeks after 24 h of MCAO. By contrast, the rats in the sham group and the model group were reared in standard cages. Before the rats were sacrificed at the 3rd, 7th and 14th d after MCAO, their neurological functions were tested using modified neurological severity scores ( mNSS) , and the mRNA and protein levels of HSP70 and C-MYC were detected using a reverse transcriptase-polymerase chain reaction (RT-PCR) , immunohistochemistry and Western blotting. Results Neurological function in the exercise group at the 7th and 14th days after MCAO had improved significantly compared with the control and model groups.The mRNA and protein levels of HSP70 and C-MYC were significantly upregulated at the 7th and 14th days. Conclusions Treadmill training can improve neurological function by upregulating the expression of HSP70 and C-MYC in the ischemic brain after MCAO.
