中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2012年
8期
1514-1515
,共2页
韩兆峰%周健%魏爱周%郭鹏飞%张树堂
韓兆峰%週健%魏愛週%郭鵬飛%張樹堂
한조봉%주건%위애주%곽붕비%장수당
脂肪干细胞%表皮细胞%诱导%分化
脂肪榦細胞%錶皮細胞%誘導%分化
지방간세포%표피세포%유도%분화
Adipose-derived stem cells%Epidermal cells%Induction%Differentiation
目的 比较两种不同方法培养诱导脂肪干细胞(ASCs)向表皮细胞分化效果的差异,以寻找更好的诱导分化方法.方法 利用Transwell装置共培养HaCaT细胞与ASCs为共同培养组；在ASCs培养液中加入表皮生长因子(EGF)进行诱导培养为EGF诱导组；两种方法培养3、6d后的ASCs通过进行表皮细胞标志物角蛋白-19(CK-19)、β1整合素和广谱细胞角蛋白Pan-cytokeratin(Pan-CK)染色检测并计数.结果 共培养法诱导3d后的ASCs细胞CK-19、β1整合素、Pan-CK阳性率分别为(25.8±4.1)％、(29.2±3.9)％、(18.3±2.7)％,明显高于EGF诱导组的细胞阳性率,差异有统计学意义(P＜0.05).共培养法诱导6d后的ASCs细胞标记物CK-19、β1整合素、Pan-CK阳性率分别为(34.1±5.7)％、(42.8±4.3)％、(29.4±3.3)％,显著高于EGF诱导组的细胞阳性率(P＜0.01).结果提示采用共同培养法获得的表皮细胞数目多于EGF诱导组,差异有统计学意义(P＜0.05).结论 与HaCaT细胞共同培养诱导比单纯应用EGF诱导能够更好地促进ASCs向表皮细胞的转化.
目的 比較兩種不同方法培養誘導脂肪榦細胞(ASCs)嚮錶皮細胞分化效果的差異,以尋找更好的誘導分化方法.方法 利用Transwell裝置共培養HaCaT細胞與ASCs為共同培養組；在ASCs培養液中加入錶皮生長因子(EGF)進行誘導培養為EGF誘導組；兩種方法培養3、6d後的ASCs通過進行錶皮細胞標誌物角蛋白-19(CK-19)、β1整閤素和廣譜細胞角蛋白Pan-cytokeratin(Pan-CK)染色檢測併計數.結果 共培養法誘導3d後的ASCs細胞CK-19、β1整閤素、Pan-CK暘性率分彆為(25.8±4.1)％、(29.2±3.9)％、(18.3±2.7)％,明顯高于EGF誘導組的細胞暘性率,差異有統計學意義(P＜0.05).共培養法誘導6d後的ASCs細胞標記物CK-19、β1整閤素、Pan-CK暘性率分彆為(34.1±5.7)％、(42.8±4.3)％、(29.4±3.3)％,顯著高于EGF誘導組的細胞暘性率(P＜0.01).結果提示採用共同培養法穫得的錶皮細胞數目多于EGF誘導組,差異有統計學意義(P＜0.05).結論 與HaCaT細胞共同培養誘導比單純應用EGF誘導能夠更好地促進ASCs嚮錶皮細胞的轉化.
목적 비교량충불동방법배양유도지방간세포(ASCs)향표피세포분화효과적차이,이심조경호적유도분화방법.방법 이용Transwell장치공배양HaCaT세포여ASCs위공동배양조；재ASCs배양액중가입표피생장인자(EGF)진행유도배양위EGF유도조；량충방법배양3、6d후적ASCs통과진행표피세포표지물각단백-19(CK-19)、β1정합소화엄보세포각단백Pan-cytokeratin(Pan-CK)염색검측병계수.결과 공배양법유도3d후적ASCs세포CK-19、β1정합소、Pan-CK양성솔분별위(25.8±4.1)％、(29.2±3.9)％、(18.3±2.7)％,명현고우EGF유도조적세포양성솔,차이유통계학의의(P＜0.05).공배양법유도6d후적ASCs세포표기물CK-19、β1정합소、Pan-CK양성솔분별위(34.1±5.7)％、(42.8±4.3)％、(29.4±3.3)％,현저고우EGF유도조적세포양성솔(P＜0.01).결과제시채용공동배양법획득적표피세포수목다우EGF유도조,차이유통계학의의(P＜0.05).결론 여HaCaT세포공동배양유도비단순응용EGF유도능구경호지촉진ASCs향표피세포적전화.
Objective To find an optimal way to induce differentiation of adipose-derived stem cells (ASCs) into epidermal cells.Methods In co-culture group,HaCaT cells and ASCs were co-cultured by using Transwell apparatus.In epidermal growth factor (EGF) induction group,EGF was added into the culture medium of ASCs.After culture for 3 and 6 days,ASCs were detected by counting the number and dying the markers of epidermal cells such as cytokeratin 19 (CK-19),integrin β1 and pan-cytokeratin (Pan-CK).Results The positive rate of CK-19,integrin β1 and Pan-CK in ASCs was (25.8 ±4.1 )％,( 29.2 ± 3.9) ％ and ( 18.3 ± 2.7 ) ％ respectively at 3rd day after culture by co-culture method,which was significantly higher than in EGF induction group (P ＜ 0.05).The positive rate of CK-19,integrin β1 and Pan-CK in ASCs was ( 34.1 ± 5.7) ％,(42.8 ± 4.3 ) ％ and (29.4 ± 3.3 ) ％ respectively at 6th day after culturc by co culturc method,which was significantly higher than in EGF induction group ( P ＜0.01 ).The number of epidermal cells obtained by co-culture method was significantly more than that in EGF induction group.Conclusion Co-culture of ASCs with HaCaT cells is an optimal method to promote the differentiation of ASCs into epidermal cells.
