CAJ | 학술논문

目的分析急性冠状动脉综合征(ACS)和稳定性心绞痛(SAP)患者外周血中1型辅助性T细胞2型辅助性T细胞(Th1/Th2)的变化,探讨Th细胞在ACS中的变化及对血管内皮细胞损伤的作用.方法收集40例ACS患者和18例SAP患者的外周血,检测外周血中Th1/Th2相关细胞因子的浓度,及不同细胞哑群对人脐静脉内皮细胞(HUVEC)的杀伤作用.结果 ACS组外周血中干扰素-γ(IFN-γ)和白细胞介素-2(IL-2)的浓度,均高于SAP组[IFN-γ:(131.2±42.2)ng/L比(47.6±20.2)ng/L,P<0.05;IL-2:(83.7±21.3)ns/L比(46.2 ±16.7)ng/L,P<0.05],ACS组外周血中IL-10浓度低于SAP组[(16.7±4.3)ng/L比(27.5±5.5)ns/L,P<0.05];ACS组患者的外周血单个核细胞(PBMC)对HUVEC的杀伤作用较SAP组明显增强(PBMC:28.84%±4.20%比20.28%±2.71%,P<0.05),而ACS组患者清除CD4+T细胞的PBMC[(CD4-)-PBMC]对HUVEC的杀伤作用与SAP组比较,差异无统计学意义[(CD4-)-PBMC:20.70%±3.26%比20.28%±2.71%,P>0.05].结论 ACS患者Th1亚群相关细胞凶子水平明显高于SAP患者,Th2亚群相父细胞因子水平显著低于SAP患者,Th1/Th2亚群失衡在PBMC对血管内皮的损伤中起促进作用,提示Th1/Th2亚群失衡可能参与了ACS的发生.
목적 분석급성관상동맥종합정(ACS)화은정성심교통(SAP)환자외주혈중1형보조성T세포2형보조성T세포(Th1/Th2)적변화,탐토Th세포재ACS중적변화급대혈관내피세포손상적작용.방법 수집40례ACS환자화18례SAP환자적외주혈,검측외주혈중Th1/Th2상관세포인자적농도,급불동세포아군대인제정맥내피세포(HUVEC)적살상작용.결과 ACS조외주혈중간우소-γ(IFN-γ)화백세포개소-2(IL-2)적농도,균고우SAP조[IFN-γ:(131.2±42.2)ng/L비(47.6±20.2)ng/L,P<0.05;IL-2:(83.7±21.3)ns/L비(46.2 ±16.7)ng/L,P<0.05],ACS조외주혈중IL-10농도저우SAP조[(16.7±4.3)ng/L비(27.5±5.5)ns/L,P<0.05];ACS조환자적외주혈단개핵세포(PBMC)대HUVEC적살상작용교SAP조명현증강(PBMC:28.84%±4.20%비20.28%±2.71%,P<0.05),이ACS조환자청제CD4+T세포적PBMC[(CD4-)-PBMC]대HUVEC적살상작용여SAP조비교,차이무통계학의의[(CD4-)-PBMC:20.70%±3.26%비20.28%±2.71%,P>0.05].결론 ACS환자Th1아군상관세포흉자수평명현고우SAP환자,Th2아군상부세포인자수평현저저우SAP환자,Th1/Th2아군실형재PBMC대혈관내피적손상중기촉진작용,제시Th1/Th2아군실형가능삼여료ACS적발생.
Objective To observe the T helper 1 and T helper 2(Th1/Th2)balance and possible association to vascular endothelial cells iniury in patients with acute coronary syndromes(ACS).Methods Forty patients with ACS and l 8 patients with stable angina pectoris(SAP)were included in this study.The concentrations of T helper 1/T helper 2 sabsets related cytokines in plasma were evaluated bv ELISA Kits.Cytotoxic activity of peripheral blood mononuclear cells (PBMCs)or PBMCs depleted CD+ T cells against human umbilical vein endothelial cells(HUVECs)were evaluated by Cr51 cytotoxicity assay.Results Concentrations of T helper 1 related cytokines IFN-γ and IL-2 were significantly higher[IFN-γ:(131.2±42.2)ng/L vs.(47.6±20.2)ng/L;IL-2:(83.7 4±21.3)ng/L vs.(46.2±16.7)ng/I.,all P<0.05]while T helper 2 related cytokine IL-10 concentration was significantly lower[(1 6.7 ±4.3)ng/L vs.(27.5±5.5)ng/L.P<0.05 ] in patients with ACS compared to those in SAP patients.Cytotoxic activity of PBMCs against HUVECs in patients with ACS was also significantly higher than that in patients with SAP (28.84%±4.20% vs.20.28%±2.71%,P<0.05).Conclusions In patients with ACS.Th1 related cytokines were significantly upregulated while Th2 related cytokines were significantly downregulated.This imbalante of Th1/Th2 accelerated PBMCs mediated endothelium iniury in patients with ACS.