CAJ | 학술논문

目的观察生物可降解材料聚β-羟基丁酸酯(PHB)与种子细胞即兔膀胱移行上皮细胞及平滑肌细胞间的生物相容性,探讨其作为种子细胞载体构建组织工程化尿道的可行性.方法经过传代培养,分别在PHB与上皮细胞共同培养2、7 d,与平滑肌细胞共同培养1、5 d,每组各取6个样本,用噻唑蓝(MTT)比色法检测细胞的增殖情况,并利用扫描电镜检测细胞在材料上的空间生长情况,评估移行上皮细胞和平滑肌细胞与聚β-羟基丁酸酯的生物相容性.结果平滑肌细胞能够在该材料上正常生长,各组间A值差异无统计学意义(P>0.05);而移行上皮细胞在该材料上只有少量生长,且实验组与对照组间A值差异有统计学意义(P<0.05),而A、B两实验组间A值差异无统计学意义(P>0.05).结论聚β-羟基丁酸酯与平滑肌细胞具有良好的生物相容性,但其对移行上皮细胞的生长具有一定的抑制作用,生物相容性不甚理想.
목적 관찰생물가강해재료취β-간기정산지(PHB)여충자세포즉토방광이행상피세포급평활기세포간적생물상용성,탐토기작위충자세포재체구건조직공정화뇨도적가행성.방법 경과전대배양,분별재PHB여상피세포공동배양2、7 d,여평활기세포공동배양1、5 d,매조각취6개양본,용새서람(MTT)비색법검측세포적증식정황,병이용소묘전경검측세포재재료상적공간생장정황,평고이행상피세포화평활기세포여취β-간기정산지적생물상용성.결과 평활기세포능구재해재료상정상생장,각조간A치차이무통계학의의(P>0.05);이이행상피세포재해재료상지유소량생장,차실험조여대조조간A치차이유통계학의의(P<0.05),이A、B량실험조간A치차이무통계학의의(P>0.05).결론 취β-간기정산지여평활기세포구유량호적생물상용성,단기대이행상피세포적생장구유일정적억제작용,생물상용성불심이상.
Objective To observe the biocompafibility of polyhydroxybutyrate (PHB) to bladder transitional epithelial cells and smooth muscle cells and research the feasibility of PHB as a carder of seed cells to construct tissue engineered urethra. Methods At 2nd and 7th day the transitional epithelial cells were cultured with PHB in vitro,taking 6 samples. So deal with smooth muscle cells at lard and 5thd. Cell adhesion and cell proliferation were detected with MTr assay. Cells were examined by SEM to assess cell growth and morphology. Finally, evaluate the biocompatibility of PHB combined with bladder transitional epithelial cells and smooth muscle cells. Results Smooth muscle cells could adhere to PHB and grew well on it, there is no significant difference between three groups (P > 0.05). Transitional epithelial cells ad-hered to PHB difficultly, there is significant difference between experimental group and control group (P <0.05), but there is no significant difference between A group and B group of experimental group (P >0.05). Conclusion PHB has a good biocompatibility with bladder smooth muscle cells,while has a poor biocompatibility with bladder transitional epithelial cells.