CAJ | 학술논문

目的观察乙肝疫苗是否增强细胞因子诱导的杀伤细胞(cytokine-induced killer cell,CIK)对于乙型肝炎病毒转基因小鼠(hepatitis B virus transgenic mice,HBV-Tg)的抗病毒作用.方法给予HBV-Tg腹腔注射CIK细胞,皮下注射乙肝疫苗,用荧光定量PCR检测外周血中HBV DNA水平变化,流式细胞仪检测外周血中T淋巴细胞亚群,并用HE染色观察肝脏的组织病理改变.结果 CIK细胞降低了HBV-Tg鼠外周血中病毒载量,血中CD3~+、CD4~+及CD8~+细胞增多,乙肝疫苗和CIK细胞联合应用后,这种作用增强.结论乙肝疫苗增强了CIK对于HBV-Tg小鼠的治疗作用,这种增强作用是否通过增加体内CD3~+、CD4~+及CD8~+细胞,尤其是CD8~+细胞而实现,有待进一步研究.
목적 관찰을간역묘시부증강세포인자유도적살상세포(cytokine-induced killer cell,CIK)대우을형간염병독전기인소서(hepatitis B virus transgenic mice,HBV-Tg)적항병독작용.방법 급여HBV-Tg복강주사CIK세포,피하주사을간역묘,용형광정량PCR검측외주혈중HBV DNA수평변화,류식세포의검측외주혈중T림파세포아군,병용HE염색관찰간장적조직병리개변.결과 CIK세포강저료HBV-Tg서외주혈중병독재량,혈중CD3~+、CD4~+급CD8~+세포증다,을간역묘화CIK세포연합응용후,저충작용증강.결론 을간역묘증강료CIK대우HBV-Tg소서적치료작용,저충증강작용시부통과증가체내CD3~+、CD4~+급CD8~+세포,우기시CD8~+세포이실현,유대진일보연구.
Objective To observe whether hepatitis B vaccine enhance the treating effect of cyto-kine induced kill(CIK) cells on hepatitis B virus transgenic(HBV-Tg) mice. Methods The HBV-Tg mice were treated with CIK cells by peritoneal injection and hepatitis B vaccine by hypodermic injection. The HBV DNA level were tested by real-time PCR,T lymphocyte subgroup were detected by flow cytometry and the pathological diversify of hepatic tissue were observed by HE staining. Results The HBV DNA loading in peripheral blood of HBV-Tg mice decreased after CIK cells were treated and CD3~+ , CD4~+ and CD8~+ cells increased which were enhanced after CIK cells combined with hepatitis B vaccine. Conclusion Hepa-titis B vaccine enhanced the treating effect of CIK on HBV-Tg mice which may be implemented by increased the blood level of CD3~+, CD4~+ and CD8~+ cells, especially CD8~+ cells level.