中华神经医学杂志
中華神經醫學雜誌
중화신경의학잡지
CHINESE JOURNAL OF NEUROMEDICINE
2012年
3期
261-265
,共5页
刘盛泽%陈实%张永亮%林健%林茂晖%杨庆武%陈文旭%陈爱国%蒋小玲%林海谅%兰小鹏%赵猛
劉盛澤%陳實%張永亮%林健%林茂暉%楊慶武%陳文旭%陳愛國%蔣小玲%林海諒%蘭小鵬%趙猛
류성택%진실%장영량%림건%림무휘%양경무%진문욱%진애국%장소령%림해량%란소붕%조맹
高血压脑出血%脑脊液%蛋白组学%SELDI-TOF-MS技术
高血壓腦齣血%腦脊液%蛋白組學%SELDI-TOF-MS技術
고혈압뇌출혈%뇌척액%단백조학%SELDI-TOF-MS기술
Hypertensive intracerebral hemorrhage%Cerebrospinal fluid%Proteomic%SELDI-TOF-MS
目的 筛选出与高血压脑出血密切相关的疾病特异性蛋白,探讨其在高血压脑出血发病机制中的作用. 方法 应用表面增强激光解吸离子化飞行时间质谱(SELDI-TOF-MS)技术检测69例脑脊液标本的蛋白质谱,其中健康对照者15例、高血压患者10例、高血压脑出血术前患者24例、高血压脑出血术后患者20例.比较分析高血压脑出血术前组与高血压组、高血压脑出血术前组与术后组、健康对照组与高血压组脑脊液蛋白质谱,用Biomark Wizard软件分析其差异蛋白质峰,并通过蛋白数据库检索出与高血压脑出血密切相关的疾病特异性蛋白. 结果 通过比较分析高血压脑出血术前组与高血压组、高血压脑出血术前组与术后组、健康对照组与高血压组脑脊液蛋白质谱,分别筛选出23个、16个、0个差异蛋白质峰.高血压脑出血术前组与高血压组间的23个差异蛋白质峰中有6个差异峰有重要意义,与这6个差异峰相对分子质量接近的蛋白质分别为B型脑钠肽、中性粒细胞防御素2、活性氧自由基、亚型戊烯半胱氨酸氧化酶、分泌载体相关的膜蛋白SCAMP4、白介素-31. 结论 通过分析高血压脑出血患者脑脊液蛋白质谱,可以筛选出与高血压脑出血相关的疾病特异性蛋白,其能成为预测高血压脑出血的分子标志物.
目的 篩選齣與高血壓腦齣血密切相關的疾病特異性蛋白,探討其在高血壓腦齣血髮病機製中的作用. 方法 應用錶麵增彊激光解吸離子化飛行時間質譜(SELDI-TOF-MS)技術檢測69例腦脊液標本的蛋白質譜,其中健康對照者15例、高血壓患者10例、高血壓腦齣血術前患者24例、高血壓腦齣血術後患者20例.比較分析高血壓腦齣血術前組與高血壓組、高血壓腦齣血術前組與術後組、健康對照組與高血壓組腦脊液蛋白質譜,用Biomark Wizard軟件分析其差異蛋白質峰,併通過蛋白數據庫檢索齣與高血壓腦齣血密切相關的疾病特異性蛋白. 結果 通過比較分析高血壓腦齣血術前組與高血壓組、高血壓腦齣血術前組與術後組、健康對照組與高血壓組腦脊液蛋白質譜,分彆篩選齣23箇、16箇、0箇差異蛋白質峰.高血壓腦齣血術前組與高血壓組間的23箇差異蛋白質峰中有6箇差異峰有重要意義,與這6箇差異峰相對分子質量接近的蛋白質分彆為B型腦鈉肽、中性粒細胞防禦素2、活性氧自由基、亞型戊烯半胱氨痠氧化酶、分泌載體相關的膜蛋白SCAMP4、白介素-31. 結論 通過分析高血壓腦齣血患者腦脊液蛋白質譜,可以篩選齣與高血壓腦齣血相關的疾病特異性蛋白,其能成為預測高血壓腦齣血的分子標誌物.
목적 사선출여고혈압뇌출혈밀절상관적질병특이성단백,탐토기재고혈압뇌출혈발병궤제중적작용. 방법 응용표면증강격광해흡리자화비행시간질보(SELDI-TOF-MS)기술검측69례뇌척액표본적단백질보,기중건강대조자15례、고혈압환자10례、고혈압뇌출혈술전환자24례、고혈압뇌출혈술후환자20례.비교분석고혈압뇌출혈술전조여고혈압조、고혈압뇌출혈술전조여술후조、건강대조조여고혈압조뇌척액단백질보,용Biomark Wizard연건분석기차이단백질봉,병통과단백수거고검색출여고혈압뇌출혈밀절상관적질병특이성단백. 결과 통과비교분석고혈압뇌출혈술전조여고혈압조、고혈압뇌출혈술전조여술후조、건강대조조여고혈압조뇌척액단백질보,분별사선출23개、16개、0개차이단백질봉.고혈압뇌출혈술전조여고혈압조간적23개차이단백질봉중유6개차이봉유중요의의,여저6개차이봉상대분자질량접근적단백질분별위B형뇌납태、중성립세포방어소2、활성양자유기、아형무희반광안산양화매、분비재체상관적막단백SCAMP4、백개소-31. 결론 통과분석고혈압뇌출혈환자뇌척액단백질보,가이사선출여고혈압뇌출혈상관적질병특이성단백,기능성위예측고혈압뇌출혈적분자표지물.
Objective To compare the proteomic spectrum of cerebrospinal fluid between patients with hypertensive cerebral hemorrhage before surgery and patients with hypertension,between patients with hypertensive cerebral hemorrhage before surgery and after surgery,between healthy controls and patients with hypertension,and find out disease-specific proteins (DSPs) closely correlated with hypertensive cerebral hemorrhage to explore their roles in hypertensive cerebral hemorrhage. Methods Sixty-nine cerebrospinal fluid samples from patients with hypertensive cerebral hemorrhage (before operation,n=24; after operation,n=20),from patients with hypertension (n=10),and from healthy controls (n=15) were detected by surface-enhanced laser desorption/ionization time-of-flight mass spectrometry (SELDI-TOF-MS).Data were analyzed by Biomark Wizard software(Ciphergen Inc.),and DSPs closely correlated with hypertensive cerebral hemorrhage were searched by protein databases.Results By comparing and analyzing the proteomic spectrum of patients from group of hypertensive cerebral hemorrhage before surgery and group of hypertension, from groups of hypertensive cerebral hemorrhage before surgery and after surgery,from group of healthy controls and group of hypertension,the candidate protein peaks were screened,and the number of them was 23,16,and 0,respectively.The 23 different protein peaks between patients with hypertensive cerebral hemorrhage before surgery and patients with hypertension were analyzed by Biomarker pattern software, showing 6 different protein peaks were important; through searching the database according to molecular weight,we noted that these protein might be brain natriuretic peptide (BNP),human neutrophil peptide alpha-defensin (HNP-2),reactive oxygen species (ROS), PCYOX, secretory carrier membrane protein 4 (SCAMP4) and interleukin-31. Conclusion Differentially expressed proteins from patients with hypertensive cerebral hemorrhage could be screened after analyzing the map of cerebrospinal fluid protein mass spectrum,and these differentially expressed proteins are expected to be the molecular markers to forecast hypertensive cerebral hemorrhage.
