中国医师进修杂志
中國醫師進脩雜誌
중국의사진수잡지
CHINESE JOURNAL OF POSTGRADUATES OF MEDICINE
2012年
2期
28-31
,共4页
冯爱强%杨克艳%张彦武%冯浩
馮愛彊%楊剋豔%張彥武%馮浩
풍애강%양극염%장언무%풍호
乳腺肿瘤%病理学%逆转录聚合酶链反应%p130Cas%paxillin
乳腺腫瘤%病理學%逆轉錄聚閤酶鏈反應%p130Cas%paxillin
유선종류%병이학%역전록취합매련반응%p130Cas%paxillin
Breast neoplasms%Pathology%Reverse transcriptase polymerase chain reaction%p130Cas%paxillin
目的 研究p130Cas和paxillin在乳腺癌组织中的表达,及其与乳腺癌临床病理学特征的关系.方法 采用反转录-聚合酶链反应( RT-PCR)法检测30例原发性乳腺癌及15例距癌灶边缘5 cm以上经病理学检查正常的乳腺组织、乳腺纤维瘤10例乳腺组织中p130Cas和paxillinmRNA的表达情况.结果 在正常乳腺组织和乳腺纤维瘤组织中p130Cas和paxillin分别为0.444±0.088、0.493±0.073和0.739±0.092、0.755±0.137,乳腺癌组织中p130Cas和paxillih分别为0.914±0.186、0.303±0.043,与前两者比较,乳腺癌组织中p130Cas表达显著增高(P＜0.01),而paxillin表达显著降低(P＜0.01).p130Cas的表达与患者年龄(r=0.599,P=0.000)、绝经状况(t=5.602,P=0.000)、肿瘤细胞的雌激素受体(ER)和孕激素受体(PR)表达(t=5.768,P=0.000;t=4.151,P=0.000)及组织学分级(r=-0.668,P=0.000)相关,而与肿瘤大小(F=0.717,P=0.497)、淋巴结转移状况(F=1.230,P=0.308)及病理学分期(r=0.283,P=0.137)无关.paxillin的表达与患者年龄(r =0.294,P=0.115)、绝经状况(t=-0.403,P=0.690)、肿瘤细胞的ER和PR表达(t=0.749,P=0.460;t=0.006,P=0.995)及组织学分级(r=-0.173,P=0.362)无关,而与肿瘤大小(F=4.114,P=0.028)、病理学分期(r=-0.520,P=0.003)和淋巴结转移状况(F=12.418,P=0.000)有关.结论p130Cas和paxillin与乳腺癌细胞的恶性转化和侵袭、转移有关；检测上述两种因子mRNA的表达可以评价原发性及转移性乳腺癌患者的预后.
目的 研究p130Cas和paxillin在乳腺癌組織中的錶達,及其與乳腺癌臨床病理學特徵的關繫.方法 採用反轉錄-聚閤酶鏈反應( RT-PCR)法檢測30例原髮性乳腺癌及15例距癌竈邊緣5 cm以上經病理學檢查正常的乳腺組織、乳腺纖維瘤10例乳腺組織中p130Cas和paxillinmRNA的錶達情況.結果 在正常乳腺組織和乳腺纖維瘤組織中p130Cas和paxillin分彆為0.444±0.088、0.493±0.073和0.739±0.092、0.755±0.137,乳腺癌組織中p130Cas和paxillih分彆為0.914±0.186、0.303±0.043,與前兩者比較,乳腺癌組織中p130Cas錶達顯著增高(P＜0.01),而paxillin錶達顯著降低(P＜0.01).p130Cas的錶達與患者年齡(r=0.599,P=0.000)、絕經狀況(t=5.602,P=0.000)、腫瘤細胞的雌激素受體(ER)和孕激素受體(PR)錶達(t=5.768,P=0.000;t=4.151,P=0.000)及組織學分級(r=-0.668,P=0.000)相關,而與腫瘤大小(F=0.717,P=0.497)、淋巴結轉移狀況(F=1.230,P=0.308)及病理學分期(r=0.283,P=0.137)無關.paxillin的錶達與患者年齡(r =0.294,P=0.115)、絕經狀況(t=-0.403,P=0.690)、腫瘤細胞的ER和PR錶達(t=0.749,P=0.460;t=0.006,P=0.995)及組織學分級(r=-0.173,P=0.362)無關,而與腫瘤大小(F=4.114,P=0.028)、病理學分期(r=-0.520,P=0.003)和淋巴結轉移狀況(F=12.418,P=0.000)有關.結論p130Cas和paxillin與乳腺癌細胞的噁性轉化和侵襲、轉移有關；檢測上述兩種因子mRNA的錶達可以評價原髮性及轉移性乳腺癌患者的預後.
목적 연구p130Cas화paxillin재유선암조직중적표체,급기여유선암림상병이학특정적관계.방법 채용반전록-취합매련반응( RT-PCR)법검측30례원발성유선암급15례거암조변연5 cm이상경병이학검사정상적유선조직、유선섬유류10례유선조직중p130Cas화paxillinmRNA적표체정황.결과 재정상유선조직화유선섬유류조직중p130Cas화paxillin분별위0.444±0.088、0.493±0.073화0.739±0.092、0.755±0.137,유선암조직중p130Cas화paxillih분별위0.914±0.186、0.303±0.043,여전량자비교,유선암조직중p130Cas표체현저증고(P＜0.01),이paxillin표체현저강저(P＜0.01).p130Cas적표체여환자년령(r=0.599,P=0.000)、절경상황(t=5.602,P=0.000)、종류세포적자격소수체(ER)화잉격소수체(PR)표체(t=5.768,P=0.000;t=4.151,P=0.000)급조직학분급(r=-0.668,P=0.000)상관,이여종류대소(F=0.717,P=0.497)、림파결전이상황(F=1.230,P=0.308)급병이학분기(r=0.283,P=0.137)무관.paxillin적표체여환자년령(r =0.294,P=0.115)、절경상황(t=-0.403,P=0.690)、종류세포적ER화PR표체(t=0.749,P=0.460;t=0.006,P=0.995)급조직학분급(r=-0.173,P=0.362)무관,이여종류대소(F=4.114,P=0.028)、병이학분기(r=-0.520,P=0.003)화림파결전이상황(F=12.418,P=0.000)유관.결론p130Cas화paxillin여유선암세포적악성전화화침습、전이유관；검측상술량충인자mRNA적표체가이평개원발성급전이성유선암환자적예후.
Objective To explore the expression of p130Cas and paxillin in breast carcinoma and investigate the relationships of p130Cas and paxillin levels with clinical and pathological characteristics.Methods Reverse transcriptase polymerase chain reaction (RT-PCR) was applied to detect the expression of p130Cas and paxillin in tumor tissues from 30 cases of primary breast carcinoma,15 cases of normal breast tissues and 10 cases of breast fibroadenoma.Results The levels of p130Cas and paxillin were 0.444 ± 0.088,0.493 ± 0.073,0.739 ± 0.092,0.755 ± 0.137 in normal breast tissues and breast fibroadenoma tissues.The levels of p130Cas and paxillin were 0.914 ±0.186,0.303 ±0.043 in breast carcinoma tissues.Breast carcinoma tissues showed higher levels of p130Cas and lower levels of paxillin than normal breast tissues and breast fibroadenoma tissues (P ＜ 0.01 ).The expression of p130Cas was related with age (r =0.599,P =0.000 ),menopausal status (t =5.602,P =0.000 ),estrogen receptor ( ER ),progesterone receptor ( PR ) status (t =5.768,P =0.000; t =4.151,P =0.000 ) and histological grades (r =-0.668,P =0.000 ),but not related with tumor size (F =0.717,P =0.497 ),lymph node status (F =1.230,P =0.308 ) and pathological stages(r =0.283,P =0.137).The expression of paxillin was related with rumor size (F=4.114,P =0.028 ),pathological stages( r =-0.520,P =0.003),and lymph node status(F=12.418,P=0.000 ),but not related with age(r =0.294,P=0.115),menopausal status(t =-0.403,P =0.690),ER,PR status(t =0.749,P =0.460;t =0.006,P =0.995) and histological grades (r =-0.173,P =0.362).Conclusions p130Cas and paxillin have relations with the malignant transformation,invasion and metastasis of breast carcinoma.Measurement of p130Cas and paxillin may provide useful prognostic information for patients with primary or metastatic breast carcinoma.
