CAJ | 학술논문

目的探讨肾透明细胞癌组织中T细胞淋巴瘤侵袭和转移诱导蛋白l( T-lymphomainvasion and metastasis 1,Tiam1)的表达与临床病理特征及预后的关系. 方法选取原发性肾透明细胞癌组织标本107例.男78例,女29例.年龄32～87岁,平均59岁.肿瘤大小按手术切除标本测量.肿瘤最大径1.4～7.6 cm,平均4.5 cm.≤2.5 cm者23例,2.6 ～5.0 cm者65例,＞5.0 cm者19例.有淋巴结转移者46例,有血管浸润者32例.Fuhrman分级:Ⅰ级22例,Ⅱ级41例,Ⅲ级27例,Ⅳ级17例.2002 AJCC TNM分期:T129例,T244例,T3 16例,T4 18例.20例正常肾组织标本作为对照组.免疫组织化学方法检测标本中Tiam1蛋白表达,结合临床病理学资料进行分析.随访5～8年,生存分析采用Kaplan-Meier法,组间差异采用Log-rank检验. 结果 20例正常肾组织中Tiam1蛋白阳性表达率为15.0％ (3/20),肿瘤组织中Tiam1蛋白阳性表达率为73.8％ (79/107),组间差异有统计学意义(P＜0.01)；Ⅰ～Ⅱ级与Ⅲ～Ⅳ级肾癌组织中Tiam1蛋白高表达阳性率分别为47.6％( 30/63)、72.7％( 32/44),T1～T2期与T3 ～T4期肾癌组织分别为49.3％(36/73)、76.5％ (26/34),无淋巴结转移组与有淋巴结转移组分别为47.5％ (29/61)、71.7％ (33/46),无血管浸润组与有血管浸润组分别为48.0％(36/75)、81.3％(26/32),组间差异均有统计学意义(P＜0.01).Tiam1蛋白高表达组与低表达组患者术后5年生存率分别为46.8％(29/62)、84.4％( 38/45),组间差异有统计学意义(P＜0.05). 结论肾癌组织中Tiam1蛋白阳性表达率明显高于正常组织,Tiam1蛋白高表达与肾癌的分期、分级、淋巴结转移、血管浸润有关,与患者性别、年龄、肿瘤大小无相关性.检测Tiam1蛋白有助于肾癌的诊断及预后评估.
목적 탐토신투명세포암조직중T세포림파류침습화전이유도단백l( T-lymphomainvasion and metastasis 1,Tiam1)적표체여림상병리특정급예후적관계. 방법 선취원발성신투명세포암조직표본107례.남78례,녀29례.년령32～87세,평균59세.종류대소안수술절제표본측량.종류최대경1.4～7.6 cm,평균4.5 cm.≤2.5 cm자23례,2.6 ～5.0 cm자65례,＞5.0 cm자19례.유림파결전이자46례,유혈관침윤자32례.Fuhrman분급:Ⅰ급22례,Ⅱ급41례,Ⅲ급27례,Ⅳ급17례.2002 AJCC TNM분기:T129례,T244례,T3 16례,T4 18례.20례정상신조직표본작위대조조.면역조직화학방법검측표본중Tiam1단백표체,결합림상병이학자료진행분석.수방5～8년,생존분석채용Kaplan-Meier법,조간차이채용Log-rank검험. 결과 20례정상신조직중Tiam1단백양성표체솔위15.0％ (3/20),종류조직중Tiam1단백양성표체솔위73.8％ (79/107),조간차이유통계학의의(P＜0.01)；Ⅰ～Ⅱ급여Ⅲ～Ⅳ급신암조직중Tiam1단백고표체양성솔분별위47.6％( 30/63)、72.7％( 32/44),T1～T2기여T3 ～T4기신암조직분별위49.3％(36/73)、76.5％ (26/34),무림파결전이조여유림파결전이조분별위47.5％ (29/61)、71.7％ (33/46),무혈관침윤조여유혈관침윤조분별위48.0％(36/75)、81.3％(26/32),조간차이균유통계학의의(P＜0.01).Tiam1단백고표체조여저표체조환자술후5년생존솔분별위46.8％(29/62)、84.4％( 38/45),조간차이유통계학의의(P＜0.05). 결론 신암조직중Tiam1단백양성표체솔명현고우정상조직,Tiam1단백고표체여신암적분기、분급、림파결전이、혈관침윤유관,여환자성별、년령、종류대소무상관성.검측Tiam1단백유조우신암적진단급예후평고.
Objective To explore the expression of Tiaml in clear cell renal cell carcinoma and analyze its correlations to pathology of disease and prognosis.Methods The expressions of Tiam1 protein in 107 specimens of human clear cell renal cell carcinoma and 20 specimens of normal renal tissues were detected by immunohistochemical staining and its clinical significance was then analyzed.Results The expression of Tiam1 protein was higher in renal cancers than in the adjacent normal tissues ( P ＜ 0.01 ).Tiam1 protein expression rates were 47.6％ and 72.7％ in Ⅰ - Ⅱ and Ⅲ - Ⅳ tumors,while 49.3％ and 76.5％ in T1 - T2 and T3 - T4 tumors,respectively ( P ＜ 0.01 ).Expression of Tiam1 protein was higher in lymph node positive renal carcinoma tissues than in lymph node negative renal carcinoma tissues ( 71.7％ versus 47.5％,P ＜ 0.05 ).The expression of Tiam1 in carcinoma tissues showed a positive relationship with tumor vascular invasion (81.3％ versus 48.0％,P ＜ 0.01 ).In patients followed-up 5 - 8 years,Kaplan-meier analysis and the log-rank test showed that the 5-year survival was significantly different between the group of lower and higher Tiaml expression groups ( 84.4％ versus 46.8％,P ＜ 0.05 ).Conclusions The expression of Tiaml protein was higher in human primary renal carcinoma than in normal renal tissues.The positive rate of Tiam1 protein expression was related to classification,TNM stage,lymph node metastasis and vascular invasion.The detection of the expression of Tiaml protein may be helpful in the diagnosis and prognosis of renal carcinoma.