CAJ | 학술논문

2型糖尿病患者118例分为阿卡波糖治疗组(A组,58例)和未服阿卡波糖组(B组,60例),57名健康个体作为对照组(C组),以实时定量PCR测定粪便中双歧杆菌、粪肠球菌的含量.结果显示,A、B两组粪便中双歧杆菌数量明显低于C组,肠球菌数量高于C组.经4周干预治疗,A、B两组双歧杆菌数量均较基线时升高,但A组的双歧杆菌数量升高程度更加显著.Spearman单因素相关分析发现,基线时双歧杆菌与脂多糖、超敏C反应蛋白(hs-CRP)、晚期糖基化指数、体重指数(BMI)呈负相关(P＜O.05或P＜O.01)；粪肠球菌与单核细胞趋化蛋白1、脂多糖、肿瘤坏死因子α、血浆纤溶酶原激活物抑制物1、hs-CRP、BMI、-HbA1c和年龄呈正相关(均P＜0.01).干预治疗4周后,上述因子和肠道菌群的相关性全部消失.多因素逐步回归分析发现,基线BMI是双歧杆菌独立负相关因素,BMI、HbA1c和年龄是粪肠球菌的独立正相关因素.
2형당뇨병환자118례분위아잡파당치료조(A조,58례)화미복아잡파당조(B조,60례),57명건강개체작위대조조(C조),이실시정량PCR측정분편중쌍기간균、분장구균적함량.결과현시,A、B량조분편중쌍기간균수량명현저우C조,장구균수량고우C조.경4주간예치료,A、B량조쌍기간균수량균교기선시승고,단A조적쌍기간균수량승고정도경가현저.Spearman단인소상관분석발현,기선시쌍기간균여지다당、초민C반응단백(hs-CRP)、만기당기화지수、체중지수(BMI)정부상관(P＜O.05혹P＜O.01)；분장구균여단핵세포추화단백1、지다당、종류배사인자α、혈장섬용매원격활물억제물1、hs-CRP、BMI、-HbA1c화년령정정상관(균P＜0.01).간예치료4주후,상술인자화장도균군적상관성전부소실.다인소축보회귀분석발현,기선BMI시쌍기간균독립부상관인소,BMI、HbA1c화년령시분장구균적독립정상관인소.
A total of 118 patients with type 2 diabetes mellitus were divided into acarbose treatment group ( A group,n =58 ) and no acarbose treatment group ( B group,n =60),and 57 healthy subjects were used as control group (C group).The quantification of fecal bifidobacteria and enterococcus faecalis in these subjects was made by realtime PCR.The results showed that fecal bifidobacteria contents in A and B groups were lower and enterococcus faecalis contents were higher compared with C group.After four weeks of intervention,fecal bifidobacteria contents in A and B groups increased ( P＜0.01 ),especially in A group,while enterococcus faecalis contents decreased ( P＜0.05 )compared with the baseline.Univariate correlation analysis showed that bifidobacteria content was negatively associated with lipopolysaccharides(LPS),advanced glycation index,high sensitive C reactive protein ( hs-CRP),and body mass index ( BMI ) at baseline ( P＜0.05 or P＜0.01 ).The enterococcus faecalis content was positively associated with levels of monocyte chemoattractant protein-1,LPS,tumor necrosis factor-α,hs-CRP,plasminogen activator inhibitor-1,BMI,and HbA1c (P ＜0.01 ).After four weeks of intervention,the above associations disappeared.Stepwise multivariate regression showed that basal BMI,HbA1c,and age contributed to the increase in the number of enterococcus faecalis,and BMI negatively contributed to the decrease in number of bifidobacteria.