国际流行病学传染病学杂志
國際流行病學傳染病學雜誌
국제류행병학전염병학잡지
INTERNATIONAL JOURNAL OF EPIDEMIOLOGY AND INFECTIOUS DISEASE
2009年
2期
92-94
,共3页
马安%王越%郭建勋%方文%干小仙
馬安%王越%郭建勛%方文%榦小仙
마안%왕월%곽건훈%방문%간소선
囊虫病%IgG4%金标免疫渗滤法%诊断
囊蟲病%IgG4%金標免疫滲濾法%診斷
낭충병%IgG4%금표면역삼려법%진단
Cysticercosis%IgG4%Immunogold filtration assay%Diagnosis
目的 创建简易、快速、敏感的囊虫IgG4检测方法 ,研制一种快速准确的囊虫病诊断试剂.方法 以猪囊尾蚴囊液粗提液为检测用抗原,以胶体金标记抗人IgG4单克隆抗体为检测探针,采用自行设计的垂直流渗滤装置检测人血清中囊虫特异性IgG4抗体,平行检测IgG进行比较分析,并以囊虫IgG4 ELISA检测试剂为对照,评价其诊断价值.结果 用金标渗滤法检测人血清中囊虫特异性IgG4的敏感性和特异性分别为95.7%(90/94)、100.0%(50/50),与IgG检测结果 相近;与包虫病患者、裂头蚴病患者血清的交叉反应率分别为8.0%(4/25)、8.3%(4/24),低于IgG检测;未发现与肺吸虫病、血吸虫病患者血清有交叉反应.金标渗滤法快速检测囊虫IgG4在敏感件和特异性上略高于ELISA检测试剂,经x<'2>检验差异无统计学意义(x<'2>=2.77,P>0.05).结论 金标渗滤法快速榆测囊虫特异性IgG4,不仅操作简便、快速,而且敏感性高、特异性强,特别适合临床检测和现场查病,值得进一步开发应用.
目的 創建簡易、快速、敏感的囊蟲IgG4檢測方法 ,研製一種快速準確的囊蟲病診斷試劑.方法 以豬囊尾蚴囊液粗提液為檢測用抗原,以膠體金標記抗人IgG4單剋隆抗體為檢測探針,採用自行設計的垂直流滲濾裝置檢測人血清中囊蟲特異性IgG4抗體,平行檢測IgG進行比較分析,併以囊蟲IgG4 ELISA檢測試劑為對照,評價其診斷價值.結果 用金標滲濾法檢測人血清中囊蟲特異性IgG4的敏感性和特異性分彆為95.7%(90/94)、100.0%(50/50),與IgG檢測結果 相近;與包蟲病患者、裂頭蚴病患者血清的交扠反應率分彆為8.0%(4/25)、8.3%(4/24),低于IgG檢測;未髮現與肺吸蟲病、血吸蟲病患者血清有交扠反應.金標滲濾法快速檢測囊蟲IgG4在敏感件和特異性上略高于ELISA檢測試劑,經x<'2>檢驗差異無統計學意義(x<'2>=2.77,P>0.05).結論 金標滲濾法快速榆測囊蟲特異性IgG4,不僅操作簡便、快速,而且敏感性高、特異性彊,特彆適閤臨床檢測和現場查病,值得進一步開髮應用.
목적 창건간역、쾌속、민감적낭충IgG4검측방법 ,연제일충쾌속준학적낭충병진단시제.방법 이저낭미유낭액조제액위검측용항원,이효체금표기항인IgG4단극륭항체위검측탐침,채용자행설계적수직류삼려장치검측인혈청중낭충특이성IgG4항체,평행검측IgG진행비교분석,병이낭충IgG4 ELISA검측시제위대조,평개기진단개치.결과 용금표삼려법검측인혈청중낭충특이성IgG4적민감성화특이성분별위95.7%(90/94)、100.0%(50/50),여IgG검측결과 상근;여포충병환자、렬두유병환자혈청적교차반응솔분별위8.0%(4/25)、8.3%(4/24),저우IgG검측;미발현여폐흡충병、혈흡충병환자혈청유교차반응.금표삼려법쾌속검측낭충IgG4재민감건화특이성상략고우ELISA검측시제,경x<'2>검험차이무통계학의의(x<'2>=2.77,P>0.05).결론 금표삼려법쾌속유측낭충특이성IgG4,불부조작간편、쾌속,이차민감성고、특이성강,특별괄합림상검측화현장사병,치득진일보개발응용.
Objective To develop a simple,rapid and sensitive immunoassay and sero-immunological diagnostic kits for diagnosis of cysticercosis. Methods Crude extracts of cyst fluid of Cysticercns cellulosae were used as antigens and anti-human IgG4 McAb was conjugated with colloidal gold as the detecting probe. A special pad for vertical-flow filtra-tion assay was designed according to the reaction procedure to detect specific IgG4 or IgG against cyticercus in patient sera. ELISA kit for determination of cyticercus IgG4 was utilized as control to evaluate, the new rapid assay. Results The sensi-tivity and specificity of this new rapid immunoassay for specific IgG4 against cysticercus were 95.7 % (90/94) and 100.0%(50/50) respectively, which were close to the results of IgG detection. There were 8.0% and 8.3% of cross reactions with patient sera of echinococcosis and of sparganosis, lower than those of IgG detection. None cross reaction showed with other helminthiasis such as schistosomiasis and paragonimiasis infection. In compared with Cyst-IgG4 ELISA kit the sensitivity and specificity of the DIGFA were higher slightly and there were no statistical differences according to chi-square criterion( x<'2> = 2.77, P > 0.05). Conclusions This new immunoassay for detection of specific IgG4 is not only rapid and simple but also sensitivitive and specificitive. It is very useful for clinical diagnosis and field investigation of cysticercosis cellulosae.
