CAJ | 학술논문

目的探讨老龄小鼠耳蜗mtDNA缺失情况,明确老龄小鼠耳蜗mtDNA缺失的位置.方法应用套式PCR和DNA测序技术对不同月龄129/CD1杂交小鼠耳蜗31个(2月龄7个,7-10月龄16个,17-19月龄8个)进行定性及定量检测.结果 1.小鼠耳蜗mtDNA3867bp大片缺失,缺失发生在nt9103-nt12970之间,其两侧nt9089-nt9103和nt12956-nt12970为核苷酸完全相同的15bp重复序列.2. 随着小鼠月龄的增加,耳蜗mtDNA3867bp缺失发生率有明显增加的趋势,2月龄小鼠未发现缺失(0/7);17-19月龄小鼠(7/8)明显高于7-10月龄小鼠(4/16)(P<0.01).3. 缺失mtDNA/总mtDNA比值,17-19月龄小鼠明显高于7-10月龄小鼠(P<0.01).结论老龄小鼠耳蜗出现mtDNA3867bp大片缺失可能与老年性聋发生相关.
목적탐토노령소서이와mtDNA결실정황,명학노령소서이와mtDNA결실적위치.방법응용투식PCR화DNA측서기술대불동월령129/CD1잡교소서이와31개(2월령7개,7-10월령16개,17-19월령8개)진행정성급정량검측.결과 1.소서이와mtDNA3867bp대편결실,결실발생재nt9103-nt12970지간,기량측nt9089-nt9103화nt12956-nt12970위핵감산완전상동적15bp중복서렬.2. 수착소서월령적증가,이와mtDNA3867bp결실발생솔유명현증가적추세,2월령소서미발현결실(0/7);17-19월령소서(7/8)명현고우7-10월령소서(4/16)(P<0.01).3. 결실mtDNA/총mtDNA비치,17-19월령소서명현고우7-10월령소서(P<0.01).결론노령소서이와출현mtDNA3867bp대편결실가능여노년성롱발생상관.
Objectives To study the status of cochlear mitochondrial DNA (mtDNA) and to determine the location of mtDNA deletion in aged mice. Methods We detected cochlear mtDNA in 2, 7-10 and 17-19 month old mice by nested polymerase chain reaction (PCR) and DNA sequencing. Results mtDNA3867bp deletions were found in the cochleae of aged mice. The deletion occurred within nt9103-nt12970 and were flanked by 15 base pair direct repeats. Comparing the incidence of mtDNA3867bp deletions, 17-19 month old mice (7/8) were significantly higher than 7-10 month old mice (4/16). The deletion was not observed in 2 month old mice (0/7). The ratio of deleted mtDNA/total mtDNA in 17-19 month old mice was higher than in 7-10 month old mice (P<0.001). Conclusion Cochlear mtDNA 3867bp deletion in aged mice may be related to presbycusis.