吉首大学学报：自然科学版
吉首大學學報：自然科學版
길수대학학보：자연과학판
Journal of Jishou University(Natural Science Edition)
2011年
3期
78-81
,共4页
贺乐%刘祝祥%朱杰%李晓腾%陈奇辉%熊利芝
賀樂%劉祝祥%硃傑%李曉騰%陳奇輝%熊利芝
하악%류축상%주걸%리효등%진기휘%웅리지
蛇足石杉%内生真菌%ITS序列%系统发育分析%鉴定
蛇足石杉%內生真菌%ITS序列%繫統髮育分析%鑒定
사족석삼%내생진균%ITS서렬%계통발육분석%감정
Huperzia crispate%endophytic fungi%ITS gene sequence%phylogenetic analysis%identification
以蛇足石杉为材料,经过严格的表面消毒,分离纯化得到1株内生真菌JSM 10.对JSM 10进行培养特征、显微形态观察和基于ITS序列的系统发育分析.结果表明：菌株JSM 10和Fusarium属的Fusarium oxysporum菌株系统发育关系最为密切,聚在同一个小枝上,相似程度为99%.因此,从系统发育分析来看,JSM 10应为Fusarium oxysporum菌株.
以蛇足石杉為材料,經過嚴格的錶麵消毒,分離純化得到1株內生真菌JSM 10.對JSM 10進行培養特徵、顯微形態觀察和基于ITS序列的繫統髮育分析.結果錶明：菌株JSM 10和Fusarium屬的Fusarium oxysporum菌株繫統髮育關繫最為密切,聚在同一箇小枝上,相似程度為99%.因此,從繫統髮育分析來看,JSM 10應為Fusarium oxysporum菌株.
이사족석삼위재료,경과엄격적표면소독,분리순화득도1주내생진균JSM 10.대JSM 10진행배양특정、현미형태관찰화기우ITS서렬적계통발육분석.결과표명：균주JSM 10화Fusarium속적Fusarium oxysporum균주계통발육관계최위밀절,취재동일개소지상,상사정도위99%.인차,종계통발육분석래간,JSM 10응위Fusarium oxysporum균주.
Endophytic fungi JSM 10 was isolated from surface-sterilized Huperzia crispate.Phylogenetic analysis of JSM 10 was carried out based on ITS gene sequences.Phylogenetic analysis result showed that JSM 10 strain was closely related to the recognized members of the genus Fusarium,in which strain JSM 10 resembled Fusarium oxysporum at a rate of 99% and JSM 10 was identified as Fusarium oxysporum.
