CAJ | 학술논문

利用西方蜜蜂雄蜂头部5’LongSAGE文库中的一组标签序列，在蜜蜂基因组序列第9号连锁群上定位一个新的西方蜜蜂表皮蛋白基因转录起始位点，并克隆了该基因的eDNA序列，继而利用此cDNA序列分析了该基因的内含子和外显子结构．分析结果显示：该基因的DNA序列长1253bp，含有4个外显子和3个内含子，其cDNA长598bp，编码一个169AA的富含Val和Pro残基（23．67％，20．12％）多肽序列．BLAST分析发现，该蛋白与已知的4个昆虫表皮蛋白序列的相似性为47．54％，且其C末端有一个共有基序，说明这5个蛋白可能属于同一个表皮蛋白家族．该研究结果提供了一个新的蜜蜂表皮蛋白基因，且该基因在雄蜂头部表达水平较高．
이용서방밀봉웅봉두부5’LongSAGE문고중적일조표첨서렬，재밀봉기인조서렬제9호련쇄군상정위일개신적서방밀봉표피단백기인전록기시위점，병극륭료해기인적eDNA서렬，계이이용차cDNA서렬분석료해기인적내함자화외현자결구．분석결과현시：해기인적DNA서렬장1253bp，함유4개외현자화3개내함자，기cDNA장598bp，편마일개169AA적부함Val화Pro잔기（23．67％，20．12％）다태서렬．BLAST분석발현，해단백여이지적4개곤충표피단백서렬적상사성위47．54％，차기C말단유일개공유기서，설명저5개단백가능속우동일개표피단백가족．해연구결과제공료일개신적밀봉표피단백기인，차해기인재웅봉두부표체수평교고．
In this study,we mapped the transcription start site （TSS） of a novel cuticular protein gene from Apis mellifera with a group of 5＇ LongSAGE tag sequences, and cloned its full-length eDNA sequence. The genomic DNA sequence of this novel gene is 1 253 bp,containing four exons and three introns. Its full-length eDNA is 598 bp, encoding a 169 AA polypeptide sequence abundant of Val and Pro residues（23. 67%, 20. 12%）. Multialignment analysis reveals that the similarity of deduced amino acid sequence with four reported insect cutieular proteins is 47. 54%, and shares a common motif at C-terminal of the cuticular protein , indicating they are likely to be a member of a euticular protein family. Our 5＇ LongSAGE data show this gene is highly expressed in drone head of this study. This results of this study provided a novel cutieular protein gene of honeybee.