CAJ | 학술논문

实验以枯草芽孢杆菌BS-1101原生质体的制备率为目的,通过中心组合试验对影响枯草芽孢杆菌BS-1101原生质体的制备率的三个因素：酶解温度、酶解时间、酶浓度进行了研究评价,通过中心组合试验设计与响应面分析方法得到二次回归模型程,由回归方程得到,在酶解温度37.27℃、酶浓度为5.152 mg.mL-1、酶解时间88.8 min的条件下得到枯草芽孢杆菌BS-1101制备率为77.02%,与方程预测值为较接近。结果表明利用响应面法能够很好地对枯草芽孢杆菌原生质体制备条件进行优化。对于枯草芽孢杆菌原生质体制备条件的优化有一定参考价值。
실험이고초아포간균BS-1101원생질체적제비솔위목적,통과중심조합시험대영향고초아포간균BS-1101원생질체적제비솔적삼개인소：매해온도、매해시간、매농도진행료연구평개,통과중심조합시험설계여향응면분석방법득도이차회귀모형정,유회귀방정득도,재매해온도37.27℃、매농도위5.152 mg.mL-1、매해시간88.8 min적조건하득도고초아포간균BS-1101제비솔위77.02%,여방정예측치위교접근。결과표명이용향응면법능구흔호지대고초아포간균원생질체제비조건진행우화。대우고초아포간균원생질체제비조건적우화유일정삼고개치。
The central composite design combined with response surface methodology was employed to optimize the protoplast formation conditions of Bacillus subtilis BS-1101.The critical parameters,such as lysozyme concentration,temperature,and contact time which affects the formation of protoplast of the Bacillus subtilis BS-1101 were studied using a novel technique named response surface methodology.Second-order multiple regression analysis was used to build a predicted model of the combined effects of independent variables（enzyme concentration,temperature and effect time） for maximum the yield of protoplast of Bacillus subtilis BS-1101.The optimal parameters to obtain the highest protoplast yield were as followed： temperature was 37.27 ℃,effect time was 88.8min,enzyme concentration was 5.152 mg·mL-1.The average experimental protoplast yield under the optimum conditions was found to be 77.02%,which agreed with the predicted value.The proposed method showed high degree of reproducibility.The central composite design was employed to optimize the protoplast formation conditions of Bacillus subtilis BS-1101.