福建畜牧兽医
福建畜牧獸醫
복건축목수의
FUJIAN JOURNAL OF ANIMAL HUSBANDRY AND VETERINARY
2011年
1期
27-29
,共3页
香猪%睾丸%发育%形态学
香豬%睪汍%髮育%形態學
향저%고환%발육%형태학
Xiang Pig Testis Epididymis Morphology
为了解香猪睾丸发育过程中的形态学变化,外科手术取30、40、50、60、90、110日龄（每个年龄组n=3～4）香猪右侧睾丸经中性多聚甲醛固定,石蜡包埋,组织切片采用HE染色,40倍物镜下观察细胞数量变化,经显微照相后用Scion image软件测量生精小管、输出小管和附睾管的管壁面积。结果发现30～50日龄时睾丸生精小管和管壁面积无显著变化（P〉0.05）,从60日龄开始到110日龄出现显著性扩大（P〈0.05）,输出小管、附睾管及其管壁面积均随着日龄增长而增加（P〈0.05）。30、40和50日龄时睾丸输出小管和附睾管的细胞数量变化并不显著（P〉0.05）,而从60日龄开始,睾丸输出小管和附睾管的细胞数量大量增加（P〈0.05）。30、40、50日龄睾丸生精小管中只发现有少量生殖细胞,但60～110日龄的睾丸生殖细胞数量快速增多（P〈0.05）。生精小管与输出小管管壁单位面积细胞数均随日龄增长而增加（P〈0.05）,而在附睾管出现下降（P〈0.05）。
為瞭解香豬睪汍髮育過程中的形態學變化,外科手術取30、40、50、60、90、110日齡（每箇年齡組n=3～4）香豬右側睪汍經中性多聚甲醛固定,石蠟包埋,組織切片採用HE染色,40倍物鏡下觀察細胞數量變化,經顯微照相後用Scion image軟件測量生精小管、輸齣小管和附睪管的管壁麵積。結果髮現30～50日齡時睪汍生精小管和管壁麵積無顯著變化（P〉0.05）,從60日齡開始到110日齡齣現顯著性擴大（P〈0.05）,輸齣小管、附睪管及其管壁麵積均隨著日齡增長而增加（P〈0.05）。30、40和50日齡時睪汍輸齣小管和附睪管的細胞數量變化併不顯著（P〉0.05）,而從60日齡開始,睪汍輸齣小管和附睪管的細胞數量大量增加（P〈0.05）。30、40、50日齡睪汍生精小管中隻髮現有少量生殖細胞,但60～110日齡的睪汍生殖細胞數量快速增多（P〈0.05）。生精小管與輸齣小管管壁單位麵積細胞數均隨日齡增長而增加（P〈0.05）,而在附睪管齣現下降（P〈0.05）。
위료해향저고환발육과정중적형태학변화,외과수술취30、40、50、60、90、110일령（매개년령조n=3～4）향저우측고환경중성다취갑철고정,석사포매,조직절편채용HE염색,40배물경하관찰세포수량변화,경현미조상후용Scion image연건측량생정소관、수출소관화부고관적관벽면적。결과발현30～50일령시고환생정소관화관벽면적무현저변화（P〉0.05）,종60일령개시도110일령출현현저성확대（P〈0.05）,수출소관、부고관급기관벽면적균수착일령증장이증가（P〈0.05）。30、40화50일령시고환수출소관화부고관적세포수량변화병불현저（P〉0.05）,이종60일령개시,고환수출소관화부고관적세포수량대량증가（P〈0.05）。30、40、50일령고환생정소관중지발현유소량생식세포,단60～110일령적고환생식세포수량쾌속증다（P〈0.05）。생정소관여수출소관관벽단위면적세포수균수일령증장이증가（P〈0.05）,이재부고관출현하강（P〈0.05）。
To investigate the morphology in testis development of male xiang pig.30、40、50、60、90and110-day-old pig （number=3~4） right testis were collected by surgical operation.The collected tissues were fixed in 4% （w/v） paraformaldehyde for 24 h and then processed to paraffin wax.Five-micron-thick sections were cut for staining by HE and cell number was observed with micscop.The area of seminiferous and epididymis tubule was measured with Scion image software after micrography.Sertoli cell were studied by immunohistochemistry using a specific monoclonal antibody to the GATA-4.Antibody could recognize its specific protein of sertoli cell and couldn＇t recognize its specific protein of germ cell in tissues and accounting the number of sertoli cells and germ cells in the testis.The results showed that the area of seminiferous tubule range from（6 409.97±485.38）to（7 873.62±1 359.06）μm2at 30~50day（P0.05）.It rose to（23 683.16±4 141.17）μm2 at 60 d and significantly higther than that of 30~50 d（P0.05）,then it continuing significantly increasing until 110d（24 879.18±4 193.88）μm2（P0.05）.The area of epididymis and efferente tubules were increasing from 30 d until 110 d（P0.05）.The number of sertoli cell were not changed from 30 d （32.58±5.74） to 110 d （28.21±3.36） （P0.05）.The number of germ cell didn＇t change at 30~50 d（24.10±2.66）~（26.23±3.36）.but they were significantly increasing from 60 d（140.50±23.39） to 110 d（259.60±28.03） （P0.05）.
