CAJ | 학술논문

应用聚丙烯酰胺凝胶电泳结合生化染色方法分别对雌雄泰山赤鳞鱼11种组织中苹果酸脱氢酶(Malate dehydrogenase,MDH,E.C.1.1.1.37)同工酶酶谱进行了分析.首次发现苹果酸脱氢酶、酸性磷酸酶和超氧化物岐化酶表型在赤鳞鱼雌性和雄性之间存在差异,而在同一性别不同个体之间无差异.因此,苹果酸脱氢酶、酸性磷酸酶和超氧化物岐化酶的电泳酶谱可以作为一项指标用于泰山办鳞鱼性别和组织的鉴定.这将为赤磷鱼种群的发育遗传学、品种改良和定向育种提供基础资料,从而有利于这一名贵育鱼种的开发和保护.
응용취병희선알응효전영결합생화염색방법분별대자웅태산적린어11충조직중평과산탈경매(Malate dehydrogenase,MDH,E.C.1.1.1.37)동공매매보진행료분석.수차발현평과산탈경매、산성린산매화초양화물기화매표형재적린어자성화웅성지간존재차이,이재동일성별불동개체지간무차이.인차,평과산탈경매、산성린산매화초양화물기화매적전영매보가이작위일항지표용우태산판린어성별화조직적감정.저장위적린어충군적발육유전학、품충개량화정향육충제공기출자료,종이유리우저일명귀육어충적개발화보호.
Polyacrylamide gel electrophoresis (PAGE) and biochemical staining method were used in this study for the analysis on malate dehydrogenase (MDH,E.C.1.1.1.37) isozymes zymogram in 11 different types of tissues of male and female Varicorhinus macrolepis.It had been found for the first time that the phenotype of malate dehydrogenase (MDH),acid phosphatase (ACP) and superoxide dismutase (SOD) showed difference between male and female V.macrolepis,and there was no difference among different individuals in the same sex.Therefore,the electrophoresis band of malate dehydrogenase,acid phosphatase and superoxide dismutase could be used as an indicator for the identification of gender and tissues of V.macrolepis,which would provide basic data for the developmental genetics,vadety improvement and directed breeding of V.macrolepis groups,thus facilitating the development and protection of this valuable fish species.