中西医结合学报
中西醫結閤學報
중서의결합학보
JOURNAL OF CHINESE INTEGRATIVE MDEICINE
2010年
2期
111-115
,共5页
梅武轩%劳绍贤%余娜%周正%黄烈平%胡斌
梅武軒%勞紹賢%餘娜%週正%黃烈平%鬍斌
매무헌%로소현%여나%주정%황렬평%호빈
胃炎%水通道蛋白%基因表达%湿热证%荧光定量聚合酶链反应
胃炎%水通道蛋白%基因錶達%濕熱證%熒光定量聚閤酶鏈反應
위염%수통도단백%기인표체%습열증%형광정량취합매련반응
gastritis%aquaporin%gene expression%dampness-heat syndrome%fluorescence quantitative polymerase chain reaction
目的:探讨慢性浅表性胃炎(chronic superficial gastritis, CSG)不同程度脾胃湿热证与胃黏膜水通道蛋白(aquaporin, AQP)3、4基因表达的相关性.方法:共纳入24例CSG患者,按脾胃湿热证不同程度分为轻、中、重度组,另选择8名健康人作为正常对照.胃镜下取胃体上部黏膜,液氮保存,荧光定量聚合酶链反应法检测胃黏膜AQP3和AQP4的基因表达.结果:中、重度脾胃湿热证组AQP3和AQP4基因表达水平均高于正常对照组和轻度脾胃湿热证组(P<0.05,P<0.01).重度脾胃湿热证组AQP3高于中度脾胃湿热证组(P<0.05).结论:脾胃湿热程度不同,胃黏膜AQP3和AQP4基因表达亦不同,脾胃湿热程度与胃黏膜AQP3和AQP4基因表达相关,其表达会随着湿热程度的加重而升高.
目的:探討慢性淺錶性胃炎(chronic superficial gastritis, CSG)不同程度脾胃濕熱證與胃黏膜水通道蛋白(aquaporin, AQP)3、4基因錶達的相關性.方法:共納入24例CSG患者,按脾胃濕熱證不同程度分為輕、中、重度組,另選擇8名健康人作為正常對照.胃鏡下取胃體上部黏膜,液氮保存,熒光定量聚閤酶鏈反應法檢測胃黏膜AQP3和AQP4的基因錶達.結果:中、重度脾胃濕熱證組AQP3和AQP4基因錶達水平均高于正常對照組和輕度脾胃濕熱證組(P<0.05,P<0.01).重度脾胃濕熱證組AQP3高于中度脾胃濕熱證組(P<0.05).結論:脾胃濕熱程度不同,胃黏膜AQP3和AQP4基因錶達亦不同,脾胃濕熱程度與胃黏膜AQP3和AQP4基因錶達相關,其錶達會隨著濕熱程度的加重而升高.
목적:탐토만성천표성위염(chronic superficial gastritis, CSG)불동정도비위습열증여위점막수통도단백(aquaporin, AQP)3、4기인표체적상관성.방법:공납입24례CSG환자,안비위습열증불동정도분위경、중、중도조,령선택8명건강인작위정상대조.위경하취위체상부점막,액담보존,형광정량취합매련반응법검측위점막AQP3화AQP4적기인표체.결과:중、중도비위습열증조AQP3화AQP4기인표체수평균고우정상대조조화경도비위습열증조(P<0.05,P<0.01).중도비위습열증조AQP3고우중도비위습열증조(P<0.05).결론:비위습열정도불동,위점막AQP3화AQP4기인표체역불동,비위습열정도여위점막AQP3화AQP4기인표체상관,기표체회수착습열정도적가중이승고.
Objective: To explore the relationship between gene expressions of aquaporin (AQP) 3 and 4 and various degrees of spleen-stomach dampness-heat syndrome in chronic superficial gastritis (CSG).Methods: Twenty-four CSG patients were divided into three groups according to the degrees of spleen-stomach dampness-heat syndrome: mild, moderate and severe groups. There were 8 patients in each group, and another 8 healthy persons were selected as normal control. Samples of mucosa of upper stomach in the included patients and normal persons were collected by gastroscopy, and then were stored in liquid nitrogen. The gene expressions of AQP3 and AQP4 in gastric tissue were examined by fluorescence quantitative polymerase chain reaction.Results: The gene expressions of AQP3 and AQP4 in the moderate and severe groups were higher than that in the mild group and normal group (P<0.05, P<0.01). The gene expression of AQP3 in the severe group was higher than that in the moderate group (P<0.05). Conclusion: The gene expressions of AQP3 and AQP4 in gastric mucosa are different in patients with various degrees of spleen-stomach dampness-heat syndrome of CSG. There is a relationship between expressions of AQP3 and AQP4 genes and the degrees of spleen-stomach dampness-heat syndrome, and the gene expressions will increase with the aggravation of the dampness-heat syndrome.