华中科技大学学报（医学版）
華中科技大學學報（醫學版）
화중과기대학학보（의학판）
ACTA UNIVERSITATIS MEDICINAE TONGJI
2009年
5期
641-644
,共4页
姚杰%张涛%曹文淼%刘伟%任精华%伍钢
姚傑%張濤%曹文淼%劉偉%任精華%伍鋼
요걸%장도%조문묘%류위%임정화%오강
肿瘤干细胞%CD133/Prominin-1%脑胶质瘤%恶性黑色素瘤%反义寡核苷酸
腫瘤榦細胞%CD133/Prominin-1%腦膠質瘤%噁性黑色素瘤%反義寡覈苷痠
종류간세포%CD133/Prominin-1%뇌효질류%악성흑색소류%반의과핵감산
cancer stem cells%CD133/Prominin-1%glioma%malignant melanoma%antisense oligonucleotides
目的 分析肿瘤干细胞表面标记物CD133/Prominin-1的功能对人脑胶质瘤及恶性黑色素瘤肿瘤细胞体外生长特性的影响.方法 选取人脑胶质瘤细胞U251及人恶性黑色素瘤细胞A357为对象,采用脂质体介导CD133/Prominin-1反义寡核苷酸(ASODN)及无关寡核苷酸(NSODN)分别转染细胞.转染后采用RT-PCR检测CD133/Prom-inin-1 mRNA水平,同时接种各组细胞,转染后第14天观察细胞生长情况.结果 在A357细胞中,CD133/Prominin-1ASDON转染组的CD133/Prominin-1 mRNA水平明显低于空白对照组及NSODN转染组(P<0.05),但其细胞集落形成率与其它两组比较差异并无统计学意义.在U251细胞中,CD133/Prominin-1 ASDON转染组的CD133/Prominin-1mRNA水平亦低于空白对照组及NSODN转染组,其第14天细胞数量也较其它两组明显减少(P<0.05).结论 通过反义寡核苷酸(ASODN)转染下调CD133/Prominin-1表达对人恶性黑色素瘤细胞的集落形成能力无明显影响,但可以显著抑制人脑胶质瘤细胞的体外增殖.
目的 分析腫瘤榦細胞錶麵標記物CD133/Prominin-1的功能對人腦膠質瘤及噁性黑色素瘤腫瘤細胞體外生長特性的影響.方法 選取人腦膠質瘤細胞U251及人噁性黑色素瘤細胞A357為對象,採用脂質體介導CD133/Prominin-1反義寡覈苷痠(ASODN)及無關寡覈苷痠(NSODN)分彆轉染細胞.轉染後採用RT-PCR檢測CD133/Prom-inin-1 mRNA水平,同時接種各組細胞,轉染後第14天觀察細胞生長情況.結果 在A357細胞中,CD133/Prominin-1ASDON轉染組的CD133/Prominin-1 mRNA水平明顯低于空白對照組及NSODN轉染組(P<0.05),但其細胞集落形成率與其它兩組比較差異併無統計學意義.在U251細胞中,CD133/Prominin-1 ASDON轉染組的CD133/Prominin-1mRNA水平亦低于空白對照組及NSODN轉染組,其第14天細胞數量也較其它兩組明顯減少(P<0.05).結論 通過反義寡覈苷痠(ASODN)轉染下調CD133/Prominin-1錶達對人噁性黑色素瘤細胞的集落形成能力無明顯影響,但可以顯著抑製人腦膠質瘤細胞的體外增殖.
목적 분석종류간세포표면표기물CD133/Prominin-1적공능대인뇌효질류급악성흑색소류종류세포체외생장특성적영향.방법 선취인뇌효질류세포U251급인악성흑색소류세포A357위대상,채용지질체개도CD133/Prominin-1반의과핵감산(ASODN)급무관과핵감산(NSODN)분별전염세포.전염후채용RT-PCR검측CD133/Prom-inin-1 mRNA수평,동시접충각조세포,전염후제14천관찰세포생장정황.결과 재A357세포중,CD133/Prominin-1ASDON전염조적CD133/Prominin-1 mRNA수평명현저우공백대조조급NSODN전염조(P<0.05),단기세포집락형성솔여기타량조비교차이병무통계학의의.재U251세포중,CD133/Prominin-1 ASDON전염조적CD133/Prominin-1mRNA수평역저우공백대조조급NSODN전염조,기제14천세포수량야교기타량조명현감소(P<0.05).결론 통과반의과핵감산(ASODN)전염하조CD133/Prominin-1표체대인악성흑색소류세포적집락형성능력무명현영향,단가이현저억제인뇌효질류세포적체외증식.
Objective To investigate whether the function of CD133/Prominin-1 ,the cancer stem cetls(CSCs) marker of gli-oma and malignant melanoma,can affect the in vitro growth of these two types of tumor cells. Methods Two different cancer cell lines-a human glioma cell line and a human malignant melanoma cell line were transfected with CD133/Prominin-1 anti-sense oligodeoxynucleotide (ASODN) or nonsense oligodeoxynucleotide ( NSODN) mediated by lipofectin. After transfection, CD133/Prominin-1 mRNA expression level was detected by RT-PCR. The cells in each group were collected and planted,and the in vitro growth of both two cell lines was observed. Results In the melanoma cells,the CD133/Prominin-1 mRNA expression level in the ASODN group was apparently lower than in the NSODN group and control group. But there was no significant difference in the colony forming rate between ASODN group and NSODN group,or control group. In the glioma cells,the cell number in the ASODN group was markedly less than in the other two groups. Conclusion The knock-down of CD133/Promi-nin-1 expression by ASODN transfection can not apparently change the colony forming rate of melanoma cells,but can markedly inhibit the in vitro growth of glioma cells.
