CAJ | 학술논문

报告基因包括lux、gfp、lacZ、inaZ、luc、cat、xylE及uidA等,其中土壤质量监测中最常用的报告基因有lux、gfp、lacZ和inaZ 4种.从不同角度比较了土壤质量监测中最常用的上述4种报告基因,简要阐述了应用于土壤质量监测中的微生物监测报告基因的定义、类别、性质及特点,展示了报告基因表达系统的构建方式与检测方法,总结了报告基因技术在监测土壤重金属、污染物质、营养物质等化学物质以及在土壤微生物及其活动、土壤根际微生物与土壤中原生动物、植物间的相互作用等方面的应用.讨论了目前报告基因技术应用的局限性及未来研究方向和重点.
보고기인포괄lux、gfp、lacZ、inaZ、luc、cat、xylE급uidA등,기중토양질량감측중최상용적보고기인유lux、gfp、lacZ화inaZ 4충.종불동각도비교료토양질량감측중최상용적상술4충보고기인,간요천술료응용우토양질량감측중적미생물감측보고기인적정의、유별、성질급특점,전시료보고기인표체계통적구건방식여검측방법,총결료보고기인기술재감측토양중금속、오염물질、영양물질등화학물질이급재토양미생물급기활동、토양근제미생물여토양중원생동물、식물간적상호작용등방면적응용.토론료목전보고기인기술응용적국한성급미래연구방향화중점.
Reporter genes include lux, gfp, lacZ, inaZ, luc, cat, xylE, uidA and so on, in which lux, gfp, lacZ and inaZ genes are the commonly used ones in the monitoring of soil quality. This review firstly compares the above-mentioned four kinds of reporter genes generally used in the monitoring of soil quality from different perspectives, and describes the definition, classifications, characteristics and feature of these reporter genes. This review also reveals the construction of the reporter gene expression system and its detection method, and then summarizes the application of the reporter gene technology in the monitoring of heavy metals, pollutants, nutrients and other chemical compounds in soil, and microbes or their activities, the interactions between soil microbes and protozoan as well as plants in soil, and finally discusses the limitations of the reporter gene technology, and the direction of research and development in the future.