生物技术
生物技術
생물기술
BIOTECHNOLOGY
2009年
5期
56-59
,共4页
张少飞%李敏%邢志国%宋强%黄登宇
張少飛%李敏%邢誌國%宋彊%黃登宇
장소비%리민%형지국%송강%황등우
粘质沙雷氏菌%16S rDNA%抑菌活性%发酵产物%稳定性
粘質沙雷氏菌%16S rDNA%抑菌活性%髮酵產物%穩定性
점질사뢰씨균%16S rDNA%억균활성%발효산물%은정성
Serratia marcescens%16S rDNA%antibacterial activity%fermentation products%stability
目的:对1株产抑菌物质的中华稻蝗内生菌SDLH进行鉴定,并对其发酵产物稳定性进行研究.方法:通过观察生长情况及菌落特征形态学、氨基酸利用、糖发酵、脱羧酶反应生理等生化检测以及16S rDNA序列测定对菌株SDLH进行分类鉴定,并在不同条件(温度、pH、光照等)下测定其发酵产物的抑菌稳定性.结果:菌株SDLH符合肠杆菌科细菌的一般特征;生理生化特征均与Serratia marcescens 的特征基本一致;菌株SDLH的16S rDNA序列长度为1 457bp.Gene bank序列登录号为EU525929.其序列在1 457bp范围内与已知的模式菌株粘质沙雷氏菌(AB244291.1)16S rDNA序列部分有100%的相似性.其发酵产物在温度(40℃～60℃)下抑菌活性稳定,在高温(≥80℃)下失去活性;在酸性条件(3≤pH<7)下,抑菌活性无明显变化,在碱性条件(pH≥10)下,抑菌活性下降;光照2～6h后抑菌活性下降.结论:菌株SDLH属于沙雷氏菌属(Serratia)粘质沙雷氏菌(Serratia marces-cens),其发酵产物具有较强稳定性,可置避光、pH中性、常温环境中短期保存,经提高活性、纯化等处理后可能作为新型天然防腐剂应用于食品领域.
目的:對1株產抑菌物質的中華稻蝗內生菌SDLH進行鑒定,併對其髮酵產物穩定性進行研究.方法:通過觀察生長情況及菌落特徵形態學、氨基痠利用、糖髮酵、脫羧酶反應生理等生化檢測以及16S rDNA序列測定對菌株SDLH進行分類鑒定,併在不同條件(溫度、pH、光照等)下測定其髮酵產物的抑菌穩定性.結果:菌株SDLH符閤腸桿菌科細菌的一般特徵;生理生化特徵均與Serratia marcescens 的特徵基本一緻;菌株SDLH的16S rDNA序列長度為1 457bp.Gene bank序列登錄號為EU525929.其序列在1 457bp範圍內與已知的模式菌株粘質沙雷氏菌(AB244291.1)16S rDNA序列部分有100%的相似性.其髮酵產物在溫度(40℃～60℃)下抑菌活性穩定,在高溫(≥80℃)下失去活性;在痠性條件(3≤pH<7)下,抑菌活性無明顯變化,在堿性條件(pH≥10)下,抑菌活性下降;光照2～6h後抑菌活性下降.結論:菌株SDLH屬于沙雷氏菌屬(Serratia)粘質沙雷氏菌(Serratia marces-cens),其髮酵產物具有較彊穩定性,可置避光、pH中性、常溫環境中短期保存,經提高活性、純化等處理後可能作為新型天然防腐劑應用于食品領域.
목적:대1주산억균물질적중화도황내생균SDLH진행감정,병대기발효산물은정성진행연구.방법:통과관찰생장정황급균락특정형태학、안기산이용、당발효、탈최매반응생리등생화검측이급16S rDNA서렬측정대균주SDLH진행분류감정,병재불동조건(온도、pH、광조등)하측정기발효산물적억균은정성.결과:균주SDLH부합장간균과세균적일반특정;생리생화특정균여Serratia marcescens 적특정기본일치;균주SDLH적16S rDNA서렬장도위1 457bp.Gene bank서렬등록호위EU525929.기서렬재1 457bp범위내여이지적모식균주점질사뢰씨균(AB244291.1)16S rDNA서렬부분유100%적상사성.기발효산물재온도(40℃～60℃)하억균활성은정,재고온(≥80℃)하실거활성;재산성조건(3≤pH<7)하,억균활성무명현변화,재감성조건(pH≥10)하,억균활성하강;광조2～6h후억균활성하강.결론:균주SDLH속우사뢰씨균속(Serratia)점질사뢰씨균(Serratia marces-cens),기발효산물구유교강은정성,가치피광、pH중성、상온배경중단기보존,경제고활성、순화등처리후가능작위신형천연방부제응용우식품영역.
Objective:Identification for the endophyte of the Oxya chinensis,the SDLH,which can produce the antibacterial substance,and analysis for the stability of the ferment substance.Method: Through the observation of the growth and morphologic characteristic of the colony,usage of the amino acid,ferment of the glucide,decarboxylase reaction and determination of the sequence of the 16S rDNA in order to identify and classify the SDLH.The anti-fungal stability under different conditions(temperature,pH,illumination) were determined.Result: The SDLH has the general character of the Enterobacteriaceae,the physiological-biochemical characteristics are identical with Serratia marcescens.The length of the 16S rDNA is 1457bp,accession number of the Genbank is EU525939.The 1457 bp of the sequence has 100% similarity with the 16S rDNA of the Serratia marcescens(AB244291.1).The ferment substance has stable anti-bacterial stabilities under temperature(40℃～60℃),and lost the activity under high temperature(≥80℃);has no obvious activity change under the acid environment(3≤pH<7),and decreased under alkalescence environment(pH≥10);decreased after illumination for 2～6 hours.Conclusion: The SDLH belongs to the Serratia marcescens of Serratia,the ferment substance has strong stability,and can be conserved under the environment of lucifuge,neutral pH and moderate room temperature.It can be used as a new kind of natural antiseptic for the food industry after the treatment of increase the activity and purification.
