CAJ | 학술논문

根据EST数据库拼接了1条大豆Cl~-通道基因的EST序列,并通过RT-PCR技术从大豆cDNA中获得该EST.Southern blot、半定量Northern blot、RT-PCR和分光光度法等检测结果表明:该EST所在Cl~-通道基因在供试栽培大豆和野生大豆杂交组合(Jackson×BB52)亲本及其耐盐杂交后代JB185(F_5)株系等材料中的拷贝数无差异,但在盐胁迫下,栽培大豆Jackson幼苗根和叶中该基因的转录水平表现减弱趋势,而野生大豆BB52和杂交后代JB185(F_5)表现增强趋势或相对较高水平,在JB185(F_5)中更为明显.盐胁迫下Jackson幼苗体内积累的Cl~-相对较多,且向叶片运输比例较大,而BB52和JB185(F_5)体内积累的Cl~-相对较少,且主要积累在根部,向叶片运输比例较小.
근거EST수거고병접료1조대두Cl~-통도기인적EST서렬,병통과RT-PCR기술종대두cDNA중획득해EST.Southern blot、반정량Northern blot、RT-PCR화분광광도법등검측결과표명:해EST소재Cl~-통도기인재공시재배대두화야생대두잡교조합(Jackson×BB52)친본급기내염잡교후대JB185(F_5)주계등재료중적고패수무차이,단재염협박하,재배대두Jackson유묘근화협중해기인적전록수평표현감약추세,이야생대두BB52화잡교후대JB185(F_5)표현증강추세혹상대교고수평,재JB185(F_5)중경위명현.염협박하Jackson유묘체내적루적Cl~-상대교다,차향협편운수비례교대,이BB52화JB185(F_5)체내적루적Cl~-상대교소,차주요적루재근부,향협편운수비례교소.
One EST (expressed sequence tag) of chloride channel genes (CLCs) in soybean was assembled according to the EST database, and was confirmed in soybean cDNA by RT-PCR technique. No difference in gene copy number of the EST among the Glycine max (Jackson), Glycine soja (BB52) and their hybrid F_5 (strain JB185) was showed by Southern blot, and its weakening transcriptional level by semi-quantitative Northern blot and RT-PCR exhibited in roots and leaves of Jackson seedlings under salt stress, while an enhanced or relatively higher trend was found in salt-stressed BB52 and JB185 seedlings, especially in JB185. Measured by photometric method, the higher Cl~- content in plants and its larger proportion in leaves of Jackson seedlings, and lower Cl~- content in plants and its larger proportion in roots in BB52 and JB185 seedlings were observed.