针灸推拿医学(英文版)
針灸推拿醫學(英文版)
침구추나의학(영문판)
JOURNAL OF ACUPUNCTURE AND TUINA SCIENCE
2008年
6期
331-333
,共3页
马晓芃%赵粹英%陈云飞%吴焕淦%施征%洪娴%杨玲
馬曉芃%趙粹英%陳雲飛%吳煥淦%施徵%洪嫻%楊玲
마효봉%조수영%진운비%오환감%시정%홍한%양령
灸法%血清%肿瘤%Fas%Bcl-2%基因表达%小鼠
灸法%血清%腫瘤%Fas%Bcl-2%基因錶達%小鼠
구법%혈청%종류%Fas%Bcl-2%기인표체%소서
Moxibustion Therapy%Serum%Neoplasms%Fas Antigen%Bcl-2 Protein,Mouse%Gene Expression%Mice
目的:观察小鼠艾灸血清体外对EL-4淋巴瘤细胞Fas、bcl-2mRNA及蛋白表达的影响.方法:采用小鼠艾灸血清培养EL-4淋巴瘤细胞24h后,应用原位杂交方法检测EL-4细胞Fas、bcl-2mRNA的表达,免疫细胞化学方法观察Fas、bcl-2蛋白表达.结果:与正常小鼠血清比较,艾灸血清培养EL-4细胞后,在bcl-2 mRNA及蛋白水平上,阳性细胞百分率均明显降低(P<0.05),在Fas mRNA及蛋白水平上,阳性细胞百分率均明显升高(P<0.05).结论:艾灸血清能够下调EL-4细胞bcl-2 mRNA及蛋白的表达,上调Fas mRNA及蛋白的表达.
目的:觀察小鼠艾灸血清體外對EL-4淋巴瘤細胞Fas、bcl-2mRNA及蛋白錶達的影響.方法:採用小鼠艾灸血清培養EL-4淋巴瘤細胞24h後,應用原位雜交方法檢測EL-4細胞Fas、bcl-2mRNA的錶達,免疫細胞化學方法觀察Fas、bcl-2蛋白錶達.結果:與正常小鼠血清比較,艾灸血清培養EL-4細胞後,在bcl-2 mRNA及蛋白水平上,暘性細胞百分率均明顯降低(P<0.05),在Fas mRNA及蛋白水平上,暘性細胞百分率均明顯升高(P<0.05).結論:艾灸血清能夠下調EL-4細胞bcl-2 mRNA及蛋白的錶達,上調Fas mRNA及蛋白的錶達.
목적:관찰소서애구혈청체외대EL-4림파류세포Fas、bcl-2mRNA급단백표체적영향.방법:채용소서애구혈청배양EL-4림파류세포24h후,응용원위잡교방법검측EL-4세포Fas、bcl-2mRNA적표체,면역세포화학방법관찰Fas、bcl-2단백표체.결과:여정상소서혈청비교,애구혈청배양EL-4세포후,재bcl-2 mRNA급단백수평상,양성세포백분솔균명현강저(P<0.05),재Fas mRNA급단백수평상,양성세포백분솔균명현승고(P<0.05).결론:애구혈청능구하조EL-4세포bcl-2 mRNA급단백적표체,상조Fas mRNA급단백적표체.
Objective: To observe the influence of moxibustion sertma of mice on the expression of Fas, bcl-2 mRNA and protein of EL-4 lymphoma cells in vitro. Method: The EL-4 lymphoma cells were cultivated for 24 h in the moxibustion serum of mice. The expression of Fas and bcl-2 mRNA of EL-4 lymphoma cells were detected by in-situ hybridization method, and the protein expression of Fas and bcl-2 were observed by the immuocytochemistry method. Results: The expression of bcl-2 mRNA and protein decreased, and the expression of Fas rnRNA and protein increased significantly in EL-4 cells, which were cultivated in the moxibustion serum compared those cultivated in normal mice serum (P<0.05). Conclusion: Moxibustion serum could down-regulate the bcl-2 mRNA and protein and up-regulate the Fas mRNA and protein of EL-4 cells.
