CAJ | 학술논문

采用典型群随机抽样在陕西省白水县抽取同羊样本.采用淀粉凝胶和醋酸纤维薄膜检测12个的结构基因座位的遗传多型性,结果在同羊中发现10个多型座位:运铁蛋白(Tf)、碱性磷酸酶(Alp)、亮氨酸氨肽酶(Lap)、芳基酯酶(Ary-Es)、血红蛋白β(Hb-β)、X-蛋白(X-p)、碳酸酐酶(CA)、过氧化氢酶(Cat)、苹果酸脱氢酶(MDH)和赖氨酸(Ly);而白蛋白(Al)和后白蛋白(Po)为单态.采用遗传贴近度和系统关系聚类分析两种方法分析同羊起源及系统地位.结果表明两种方法均支持同羊属于蒙古羊系统,同羊起源于蒙古羊,这与同羊的育成史实相符.和聚类分析方法相比,遗传贴近度分析方法可以更为有效地用于中亚以东南绵羊群体的血统判别,可以更有效地反映同羊的育成过程.
채용전형군수궤추양재합서성백수현추취동양양본.채용정분응효화작산섬유박막검측12개적결구기인좌위적유전다형성,결과재동양중발현10개다형좌위:운철단백(Tf)、감성린산매(Alp)、량안산안태매(Lap)、방기지매(Ary-Es)、혈홍단백β(Hb-β)、X-단백(X-p)、탄산항매(CA)、과양화경매(Cat)、평과산탈경매(MDH)화뢰안산(Ly);이백단백(Al)화후백단백(Po)위단태.채용유전첩근도화계통관계취류분석량충방법분석동양기원급계통지위.결과표명량충방법균지지동양속우몽고양계통,동양기원우몽고양,저여동양적육성사실상부.화취류분석방법상비,유전첩근도분석방법가이경위유효지용우중아이동남면양군체적혈통판별,가이경유효지반영동양적육성과정.
This study is based on the Tong sheep obtained by the random sampling method of typical colonies in the central area of Baishui County in Shaanxi Province, China. An investigation was undertaken to clarify the gene constitution of blood protein and nonprotein types of Tong sheep. Twelve genetic markers were examined by starch-gel electrophoresis and cellulose acetate electrophoresis. Polymorphism in Tong sheep was found at the following 10 loci, transferrin (Tf), alkaline phosphatase (Alp), leucine aminopeptidase (Lap), arylesterase (Ary-Es), hemoglobin-β (Hb-β), X-protein (X-p), carbonic anhydrase (CA), catalase (Cat),malate dehydrogenase (MDH), and lysine (Ly), whereas, albumin (Al) and postalbumin (Po) loci were monomorphic. Genetic approach degree method and phylogenetic relationship clustering method were used to judge the origin and phylogenetic status of Tong sheep. Results from both methods maintained that Tong sheep belonged to the "Mongolia group", and Mongolia sheep was the origin of Tong sheep. This was also supported by the history of Tong sheep breeding. Compared to the phylogenetic relationship clustering method, the genetic approach degree method was more reliable for the extraction from East and South of Central Asia,and was more effective in reflecting the breeding course of Tong sheep.