CAJ | 학술논문

背景与目的:鼻腔鼻窦非霍奇金淋巴瘤(non-Hodgkin's lymphoma,NHL)的患病率和免疫表型组成具有地域性差异.本研究探讨中国广州地区57例鼻腔鼻窦NHL免疫表型及其与EB病毒(Epstein-Barr virus,EBV)感染的关系.方法:收集2000年4月1日至2006年10月31日中山大学肿瘤防治中心病理科57例鼻腔鼻窦NHL标本.免疫组化染色确定免疫表型,EBER原位杂交及PCR检测EBV感染情况.结果:在同期诊断的1 412例NHL中,71例(5.03%)发生于鼻腔鼻窦,其中仅有57例适用于本研究.57例鼻腔鼻窦NHL患者中,男性38例,女性19例,年龄3～75岁,中位年龄50岁;44例(77.19%)为鼻型NK/T细胞淋巴瘤,其中37例(84.09%)为EBV+/CD56+NK细胞肿瘤,7例(15.91%)为EBV+/CD56-细胞毒性T细胞表型;11例(19.30%)为B细胞淋巴瘤,其中6例为弥漫大B表型,2例为Burkitt(Burkitt样)淋巴瘤(EBV+),1例为髓外浆细胞瘤(EBV+),1例为MALT淋巴瘤(EBV-),1例为小淋巴细胞性淋巴瘤(EBV-);2例(3.51%)为外周T细胞淋巴瘤(EBV-).37例适用DNA检测的病例中,25例(67.57%)感染缺失型LMP1(del-LMP1)EBV株,12例(32.43%)感染野生型LMP1(wt-LMP1)EBV株.结论:鼻腔鼻窦NHL最常见的类型为鼻型NK/T细胞淋巴瘤,可进一步分为EBV+/CD56+NK细胞及EBV+/CD56-细胞毒性T细胞表型.NK/T细胞淋巴瘤均感染了EBV,EBV株主要为del-LMP1型.
배경여목적:비강비두비곽기금림파류(non-Hodgkin's lymphoma,NHL)적환병솔화면역표형조성구유지역성차이.본연구탐토중국엄주지구57례비강비두NHL면역표형급기여EB병독(Epstein-Barr virus,EBV)감염적관계.방법:수집2000년4월1일지2006년10월31일중산대학종류방치중심병이과57례비강비두NHL표본.면역조화염색학정면역표형,EBER원위잡교급PCR검측EBV감염정황.결과:재동기진단적1 412례NHL중,71례(5.03%)발생우비강비두,기중부유57례괄용우본연구.57례비강비두NHL환자중,남성38례,녀성19례,년령3～75세,중위년령50세;44례(77.19%)위비형NK/T세포림파류,기중37례(84.09%)위EBV+/CD56+NK세포종류,7례(15.91%)위EBV+/CD56-세포독성T세포표형;11례(19.30%)위B세포림파류,기중6례위미만대B표형,2례위Burkitt(Burkitt양)림파류(EBV+),1례위수외장세포류(EBV+),1례위MALT림파류(EBV-),1례위소림파세포성림파류(EBV-);2례(3.51%)위외주T세포림파류(EBV-).37례괄용DNA검측적병례중,25례(67.57%)감염결실형LMP1(del-LMP1)EBV주,12례(32.43%)감염야생형LMP1(wt-LMP1)EBV주.결론:비강비두NHL최상견적류형위비형NK/T세포림파류,가진일보분위EBV+/CD56+NK세포급EBV+/CD56-세포독성T세포표형.NK/T세포림파류균감염료EBV,EBV주주요위del-LMP1형.
BACKGROUND & OBJECTIVE: There are differences in the prevalence rate and composition of immunophenotypes of Sinonasal nonHodgkin's lymphoma (NHL) depending on the geography. This study was to investigate the immunophenotypes of Sinonasal NHLs and their relationship to Epstein-Barr virus (EBV) infection in Guangzhou, China. METHODS: Fiftyseven NHL samples of the sinonasal region were collected from the Department of Pathology, Cancer Center of Sun Yat-sen University from Apr. 1 , 2000 to Oct. 31 , 2006. HE staining and immunohistochemical staining were performed. Both Epstein-Barr virus-encoded small RNA (EBER) hybridization and PCR were applied to identify EBV infection. RESULTS: Seventy-one sinonasal NHLs were found in all 1 412 NHLs (71/1 412, 5.03%). Only 57 out of the 71 NHL biopsy tissues were suitable for this study. The median age of the patients was 50 years (ranged from 3 to 75 years). There were 38 males and 19 females. Forty-four sinonasal NHLs (44/57, 77.19%) were NK/T-cell lymphoma, nasal type, all of which were infected with EBV. Among them, 37 patients (84.09%) appeared to be NK-cell neoplasm (EBV+/CD56+), and 7 cases (15.91%) showed an EBV+/CD56- cytotoxic T-cell phenotype. Eleven cases (11/57, 19.30%) were B-cell lymphoma. There were 6 cases of diffuse large B-cell immunophenotype, 2 cases of Burkitt (Burkitt-like) lymphoma (EBV+), 1 case of extramedullary plasmacytoma (EBV+), 1 case of MALTlymphoma (EBV-), and 1 case of small lymphocytic lymphoma (EBV-). Only 2 cases (2/57, 3.51%; EBV-) were peripheral T-cell lymphoma, unspecified. The del-LMP1 EBV strain harbored in 25 out of 37 available DNA samples of NK/T-cell lymphoma (25/37, 67.57%); and the wt-LMP1 EBV strain was found in 12 samples (12/37, 32.43%). CONCLUSION.- The most common sinonasal NHL is the NK/T-cell lymphoma, nasal type, which can be further subclassified into NK-cell neoplasm (EBV+/CD56+) and EBV+/CD56cytotoxic T-cell phenotype. The major EBV strain in NK/T-cell lymphomas is del-LMP1 strain.