生物化学与生物物理进展
生物化學與生物物理進展
생물화학여생물물리진전
PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS
2006年
4期
388-393
,共6页
胡廷章%王维平%曹凯鸣%王喜萍
鬍廷章%王維平%曹凱鳴%王喜萍
호정장%왕유평%조개명%왕희평
水稻%F-box motif%半定量RT-PCR%RNA印迹%茉莉酸信号途径%脱落酸途径
水稻%F-box motif%半定量RT-PCR%RNA印跡%茉莉痠信號途徑%脫落痠途徑
수도%F-box motif%반정량RT-PCR%RNA인적%말리산신호도경%탈락산도경
rice%F-box motif%semi-quantitative RT-PCR%Northern blot%JA signal pathway%ABA pathway
利用Blast检索、EST分析和RT-PCR,在水稻中分离到一个与拟南芥COI1同源的新基因,命名为OsCOI1.OsCOI1编码595个氨基酸.推测的osCOI1编码蛋白有一个F-boxmotif和16个富含亮氨酸的重复序列,这与拟南芥COI1相似.OsCOI1在氨基酸水平上和COI1有很高的同源性(74%).经半定量RT-PCR法和RNA印迹分析,表明水稻中OsCOI1表达水平在经茉莉酸甲酯和脱落酸处理后呈明显变化,但不受水杨酸和乙烯的影响,说明OsCOI1可能在茉莉酸信号途径和脱落酸途径中具有特定功能.
利用Blast檢索、EST分析和RT-PCR,在水稻中分離到一箇與擬南芥COI1同源的新基因,命名為OsCOI1.OsCOI1編碼595箇氨基痠.推測的osCOI1編碼蛋白有一箇F-boxmotif和16箇富含亮氨痠的重複序列,這與擬南芥COI1相似.OsCOI1在氨基痠水平上和COI1有很高的同源性(74%).經半定量RT-PCR法和RNA印跡分析,錶明水稻中OsCOI1錶達水平在經茉莉痠甲酯和脫落痠處理後呈明顯變化,但不受水楊痠和乙烯的影響,說明OsCOI1可能在茉莉痠信號途徑和脫落痠途徑中具有特定功能.
이용Blast검색、EST분석화RT-PCR,재수도중분리도일개여의남개COI1동원적신기인,명명위OsCOI1.OsCOI1편마595개안기산.추측적osCOI1편마단백유일개F-boxmotif화16개부함량안산적중복서렬,저여의남개COI1상사.OsCOI1재안기산수평상화COI1유흔고적동원성(74%).경반정량RT-PCR법화RNA인적분석,표명수도중OsCOI1표체수평재경말리산갑지화탈락산처리후정명현변화,단불수수양산화을희적영향,설명OsCOI1가능재말리산신호도경화탈락산도경중구유특정공능.
A novel gene, which was a homologue of Arabidopsis COI1 was isolated from rice (Oryza sativa L.) by RT-PCR and designated as OsCOI1. It encoded a protein of 595 amino acids. The similar F-box motif and 16 leucine-rich repeats were found in the deduced protein OsCOI1. OsCOI1 and COI1 showed high homology (74%) at amino acid level. Semi-quantitative RT-PCR and Northern blot analysis demonstrated that the expression of OsCOI1 in rice varied obviously after treatment with MeJA and ABA but was not affected by SA and ET, suggesting that the specific function of OsCOI1 in JA signal pathway and related ABA pathway.
