CAJ | 학술논문

目的探讨N-甲基-D-天门冬氨酸(N-methyl-D-aspartate, NMDA)和非NMDA类受体在基本呼吸节律发生和调节中的作用。方法在新生SD大鼠离体延髓脑片上记录舌下神经的呼吸节律性放电活动,在改良的Krebs液中加入兴奋性氨基酸类递质及相应的拮抗剂, 观察其RRDA的影响。结果使用非NMDA受体激动剂海人酸(Kainic acid, KA)后,可见呼吸周期及呼气时间有所延长, NMDA受体激动剂对呼吸活动则没有明显影响(P>0.1);相应的拮抗剂6-氰基-7-硝基喹恶啉土卫四(6,7-dinitroquinoxaline-2, 3-dione, DNQX)和2-氨基磷酸戊酸(D-2-amino-5- phosphonopentanoic, AP5)均可使放电频率和积分幅值明显降低吸气时间显著缩短(P<0.01),DNQX同时可致呼吸周期和呼气时间明显缩短(P<0.05)。结论在哺乳动物基本呼吸节律的产生和调节中,NMDA类受体主要对呼吸活动的强度产生调节作用;而非NMDA类受体不仅可以影响呼吸的强度,同时对呼吸的频率也发挥调节作用。
목적탐토N-갑기-D-천문동안산(N-methyl-D-aspartate, NMDA)화비NMDA류수체재기본호흡절률발생화조절중적작용。방법재신생SD대서리체연수뇌편상기록설하신경적호흡절률성방전활동,재개량적Krebs액중가입흥강성안기산류체질급상응적길항제, 관찰기RRDA적영향。결과사용비NMDA수체격동제해인산(Kainic acid, KA)후,가견호흡주기급호기시간유소연장, NMDA수체격동제대호흡활동칙몰유명현영향(P>0.1);상응적길항제6-청기-7-초기규악람토위사(6,7-dinitroquinoxaline-2, 3-dione, DNQX)화2-안기린산무산(D-2-amino-5- phosphonopentanoic, AP5)균가사방전빈솔화적분폭치명현강저흡기시간현저축단(P<0.01),DNQX동시가치호흡주기화호기시간명현축단(P<0.05)。결론재포유동물기본호흡절률적산생화조절중,NMDA류수체주요대호흡활동적강도산생조절작용;이비NMDA류수체불부가이영향호흡적강도,동시대호흡적빈솔야발휘조절작용。
Objective To study the role of N-methyl-D-aspartate (NMDA) and non-NMDA receptors in the generation and modulation of basic respiratory rhythm. Methods Respiratory rhythmical discharge activity (RRDA) of the hypoglossal nerve was recorded by suction electrode on the brainstem slices isolated from the neonatal rats, and the effects of the excitatory amino acids and its antagonists on the RRDA were investigated by adding these drugs into the modified Kreb's solution perfusing the brainstem slices. Results After application of the non-NMDA receptors agonist KA, it was found that the respiratory cycle and the expiratory time were slightly lengthened, but the NMDA receptor agonist NMDA had no effect on the RRDA. Both of the mutual antagonist DNQX and AP5 remarkably decreased the discharge frequency and the integral amplitude, accompanied by the shortening of the inspiratory time; DNQA simultaneously shortened the respiratory cycle and the expiratory time. Conclusion During the generation and the modulation of the mammalian respiratory rhythm, NMDA receptors act mainly to regulate the amplitude of the respiratory activity, and the non-NMDA receptors can not only affect the respiratory amplitude but also modulate the respiratory rhythm.