CAJ | 학술논문

为探讨电刺激Agrotis segetum雄蛾触角神经是否可以作为MGC中神经元的识别手段，采用细胞内电生理记录方法，共记录34个对性信息素有反应的MGC神经元，并测试了其中12个神经元对性信息素刺激的反应，22个神经元对性信息素刺激和电刺激的反应。结果表明，MGC神经元对性信息素及电刺激的反应模式基本一致，为一种双相反应模式。两种刺激方式均能诱导出兴奋反应，电刺激得到的兴奋反应比由性信息素刺激引起的要短；MGC神经元对两种刺激的超极化反应(抑制反应)幅度影响没有显著性差别，在电刺激实验的22个神经元上，超极化反应幅度和抑制时间都与神经元本身放电频率有一定的相关性。超极化反应是在LN参与下一定的神经回路对刺激所产生的反应而形成的，这提示两种刺激所作用的神经回路应是一致的，但从整个实验过程记录到的神经元情况来看，还须进一步结合形态学实验来验证电刺激触角神经作为MGC神经元的识别手段。
위탐토전자격Agrotis segetum웅아촉각신경시부가이작위MGC중신경원적식별수단，채용세포내전생리기록방법，공기록34개대성신식소유반응적MGC신경원，병측시료기중12개신경원대성신식소자격적반응，22개신경원대성신식소자격화전자격적반응。결과표명，MGC신경원대성신식소급전자격적반응모식기본일치，위일충쌍상반응모식。량충자격방식균능유도출흥강반응，전자격득도적흥강반응비유성신식소자격인기적요단；MGC신경원대량충자격적초겁화반응(억제반응)폭도영향몰유현저성차별，재전자격실험적22개신경원상，초겁화반응폭도화억제시간도여신경원본신방전빈솔유일정적상관성。초겁화반응시재LN삼여하일정적신경회로대자격소산생적반응이형성적，저제시량충자격소작용적신경회로응시일치적，단종정개실험과정기록도적신경원정황래간，환수진일보결합형태학실험래험증전자격촉각신경작위MGC신경원적식별수단。
The PN response pattern evoked by electrical shock would be interdependent with morphological results and could be used as descriptor of neurons in some moths.In this study，intracellular recordings were performed and 34 neurons in MGC of Agrotis segetum were recorded；all those neurons responded to sex pheromone.12 neurons just tested by sex pheromone，and 22 neurons both tested by sex pheromone and electrical stimulation.Electrical stimulation elicited similar response of neuron as blend，which was one kind of biphasic response pattern consisting of a depolarization phase and followed by a hyperpolarization phase.The depolarization phase stimulated by electrical shock was very short，but it was clearly present in this and all other tested neurons.In all tested neurons stimulated by electrical shock，there were 2-10 spikes followed the stimulus artifact and 10-80 ms burst duration in the depolarization phase.So electrical stimulation elicited the frequency response of neuron as same as the pulsed response of neuron activated by blend.Hyperpolarization potential and followed inhibition duration became bigger and longer with hyperpolarizing current injection.This was different from the case in Manduca sexta，which the early inhibitory potential was reversed with only -0.2 nA of injected current.Further analysis revealed that the frequency of neuron positively correlation with the hyperpolarization potential (R=0.667，P<0.0001) and negatively correlation with inhibition duration (R=-0.709，P<0.0001).Results indicated descriptor of neurons in MGC with electrical stimulation in A.segetum should be proved by more experiments.