CAJ | 학술논문

目的：测定仓鼠科动物高原鼢鼠Myospalax b aileyi的核rDNA基因序列，为塞隆骨正品基原检定提供分子依据。方法：采用PCR直接测序技术测定高原鼢鼠18S rRNA基因核苷酸序列并作序列特征分析。[ HT5”H〗结果：高原鼢鼠的18S rRNA序列长度为1 851 bp。根据排序比较，高原鼢鼠与2种鼠科动物间的DNA序列同源性为72.04%～72.18%。结论：通过基因序列分析，DNA测序技术可成为塞隆骨正品基原检定的准确有效手段。
목적：측정창서과동물고원분서Myospalax b aileyi적핵rDNA기인서렬，위새륭골정품기원검정제공분자의거。방법：채용PCR직접측서기술측정고원분서18S rRNA기인핵감산서렬병작서렬특정분석。[ HT5”H〗결과：고원분서적18S rRNA서렬장도위1 851 bp。근거배서비교，고원분서여2충서과동물간적DNA서렬동원성 위72.04%～72.18%。결론：통과기인서렬분석，DNA측서기술가성위 새륭골정품기원검정적준학유효수단。
Objective: Sequencing the nuclear ribosomal RNA small subunit (18S r RNA) gene of Myospalax baileyi　(Cricetidae) to develop an ultimate and defi nitive means for origin identification of genuine Sailonggu. Methods: The total DNA wa s prepared from dried tail tissues. The nuclear 18S rRNA gene region was amplifi ed by PCR using a consensus primer set and its nucleotide sequence was determine d by PCR direct sequencing. The characteristic analysis of 18S rRNA sequences wa s generated usin software program Genetyx-SV/R Version 10.1. Results: The entire 18S rRNA gene region of M. baileyi spanned 1851 bp in length. Althou gh m ultiple alignment of sequence indicates that there are only lower homology (72.0 4%～72.18%)comparing with its two alias Mus musculus (GenBank Accession numb er X 00686)and Rattus norvegicus (M11188)(Muridae), their highly conservative dom ain i s located in 1020～1509 nt. There are many variable sites from upstream of 5'-e nd , which coud provide a novel information for molecular recognition of Sailonggu. Conclusion:DNA sequencing could be a useful and reliable tool in the origin identification of genuine Sailonggu.