CAJ | 학술논문

目的：建立栝楼双转化毛状根培养系统。方法：①利用根癌农杆菌Agrobacterium tumefaciens菌株C58感染栝楼Trichosanthes kirilowii.无菌苗诱导冠瘿瘤，然后用发根农杆菌Agrobacterium rhizogenes 菌株15834感染其再生植株，诱导出毛状根；②用纸电泳检测Ti和Ri质粒是否转化成功；③运用分光光度计和SDS-PAGE检测栝楼组织中所含蛋白。结果：Ti和Ri质粒转化成功并建立了栝楼双转化毛状根培养系统。结论：双转化毛状根与一般毛状根相比，具有生长更迅速等特点而蛋白含量基本一样；因此，利用它来生产天花粉蛋白更具有开发潜力。
목적：건립괄루쌍전화모상근배양계통。방법：①이용근암농간균Agrobacterium tumefaciens균주C58감염괄루Trichosanthes kirilowii.무균묘유도관영류，연후용발근농간균Agrobacterium rhizogenes 균주15834감염기재생식주，유도출모상근；②용지전영검측Ti화Ri질립시부전화성공；③운용분광광도계화SDS-PAGE검측괄루조직중소함단백。결과：Ti화Ri질립전화성공병건립료괄루쌍전화모상근배양계통。결론：쌍전화모상근여일반모상근상비，구유생장경신속등특점이단백함량기본일양；인차，이용타래생산천화분단백경구유개발잠력。
Objective:To estabish a hairy root culture system by doubletransfonmation for Trichosathes kirilowii. Method:①Crown galls were induced by direct infection of sterile seedlings of T.kirilonii with Agrobacterium tumefaciens C58, and then the hairy roots were obtained from the regenerated plants by infection with A.rhzogenes 15834;②Transformation of Ti and Ri plasmids was inspected by high-pressure-paper electrophoresis；③ The protein contents in the tissues of T.kirilowii were inspected by spectrophotometer and SDS-PAGE.Result:A hairy root culture system has been established sucessfully by double transformation with Ti and Ri plasmids in T. kirilowii.Conclusion:Compared with the ordinary hairy roots, the double transformed hairy roots grow faster but retain similar protein contents.