CAJ | 학술논문

目的：建立HPLC测定羟基喜树碱及喜树碱含量的方法。方法：样品在弱碱性条件下溶解稀释成20μg/ml的溶液。色谱柱为YWGC18柱，46mm×250mm，10μm；流动相为甲醇－水（45∶5 5）；流速为10ml/min；检测波长为266nm。结果：羟基喜树碱回收率为1002％，日间变异（2、4、8h）RSD低于18％；喜树碱回收率为997％，日间变异（2、4、8h）RSD低于20％。结论：本法分离效果好，专属性强，分析速度快，灵敏度高，可作为原料及其制剂的含量测定方法。
목적：건립HPLC측정간기희수감급희수감함량적방법 。방법：양품재약감성조건하용해희석성20μg/ml적용액。색보 주위YWGC18주，46mm×250mm，10μm；류동상위갑순－수（45∶5 5）；류속위10ml/min；검측파장위266nm。결과：간기희수감회 수솔위1002％，일간변이（2、4、8h）RSD저우18％；희수감 회수솔위997％，일간변이（2、4、8h）RSD저우20％。결론： 본법분리효과호，전속성강，분석속도쾌，령민도고，가작 위원료급기제제적함량측정방법。
OBJECTIVE： To establish a HPLC assay for the determination of hydroxycamptothecinum and camptothecinum.METHODS： The concentrations of hydroxycamptothecinum and camptothecinum were assayed on a C18 column with a mobile phase consisting of methanol－ water(45∶ 55) at a flow rate of 1.0 ml/min,and detected at 266nm.RESULTS： The mean recovery of hydroxycamptothecinum was 100.2％ ,the 2,4,8 hours precision RSD were less than 1.8％ ;The mean recovery of camptothecinum was 99.7％ ,the 2,4,8 hours precision RSD were less than 2.0％ .CONCLUSION： The HPLC method for determination of hydroxycamptothecinum and camptothecinum was rapid,sensitive and accurate.