CAJ | 학술논문

通过凝胶层析方法,对水稻籽实中的蛋白质-Cd、Pb结合形态特征及其稳定性进行了研究. 结果表明: 水稻籽实Tris-HCl提取液经Sephadex G-75分离,洗脱液得到3个紫外吸收峰,在第1、3峰处出现了Cd、Pb浓度峰,其蛋白质-Cd、Pb结合体的表观分子量为54.5KD和5.5KD. 在蒸煮加热处理后, 水稻籽实中蛋白质-Cd、Pb结合体发生变性,使分子量为54.5KD的蛋白质-Cd、Pb结合体完全破坏,分子量为5.5KD的结合体也部分地被破坏,与蛋白质结合的Cd、Pb被释放出来. 胃蛋白酶和胰蛋白酶消化处理后,也能够破坏蛋白质-Cd、Pb结合体的形态,洗脱液中Cd、Pb的浓度分布也发生了变化,在第1、3紫外吸收峰处的Cd、Pb浓度也明显降低. 另外,在第3峰以后的洗脱液中也检测到Cd、Pb的浓度.
통과응효층석방법,대수도자실중적단백질-Cd、Pb결합형태특정급기은정성진행료연구. 결과표명: 수도자실Tris-HCl제취액경Sephadex G-75분리,세탈액득도3개자외흡수봉,재제1、3봉처출현료Cd、Pb농도봉,기단백질-Cd、Pb결합체적표관분자량위54.5KD화5.5KD. 재증자가열처리후, 수도자실중단백질-Cd、Pb결합체발생변성,사분자량위54.5KD적단백질-Cd、Pb결합체완전파배,분자량위5.5KD적결합체야부분지피파배,여단백질결합적Cd、Pb피석방출래. 위단백매화이단백매소화처리후,야능구파배단백질-Cd、Pb결합체적형태,세탈액중Cd、Pb적농도분포야발생료변화,재제1、3자외흡수봉처적Cd、Pb농도야명현강저. 령외,재제3봉이후적세탈액중야검측도Cd、Pb적농도.
The protein-binding forms of Cd and Pb in polluted rice seed and their tability were studied using Sephadex chromatography. Three peaks of absorption for Tris-HCl extraction from rice on Sephadex G 75 were detected. The Cd and Pb distributed mainly in the fractions of F-⒈ and F-Ⅲ. The apparent molecular weights of proteins-Cd and Pb for F-Ⅰ and F-Ⅲ were 54.5 and 5.5KD, respectively. After the treatment of cooking, the protein-binding forms of Cd and Pb were destroyed, high molecular weight protein-binding form (54.5KD) was decomposed into low molecular weight complex (5.5KD) or tiny peptide chain. Simultaneously, bound Cd and Pb were released or from protein changed into small protein-binding forms. After the treatment of enzyme digestion, the high molecular weight protein-binding forms of Cd and Pb were decomposed the concentrations of Cd amd Pb in the elutions of first and third peak decreased markedly, and the Cd and Pb appeared in the elution of third peak later.