CAJ | 학술논문

目的：探讨凋亡相关分子TFAR19 在TF-1细胞凋亡过程中的表达及定位。方法：以TFAR19的单克隆抗体为工具，采用荧光显微镜、共聚焦激光扫描显微镜、流式细胞仪等研究手段，观察细胞凋亡过程中TFAR19分子的表达水平和细胞内定位，分析其与细胞膜磷脂酰丝氨酸(phosphatidylserine, PS)以及细胞核DNA片段化的关系。结果：TFAR19蛋白在撤除GM-CSF的TF-1细胞中表达水平显著增高，伴有明显的核转位现象，且早于PS外翻和细胞DNA片段化。结论：TFAR19蛋白的核转位是细胞凋亡更早期发生的事件之一, 这一新发现为进一步探讨TFAR19蛋白的生物学效应，以及对细胞凋亡的多方位研究提供了新的线索和思路。
목적：탐토조망상관분자TFAR19 재TF-1세포조망과정중적표체급정위。방법：이TFAR19적단극륭항체위공구，채용형광현미경、공취초격광소묘현미경、류식세포의등연구수단，관찰세포조망과정중TFAR19분자적표체수평화세포내정위，분석기여세포막린지선사안산(phosphatidylserine, PS)이급세포핵DNA편단화적관계。결과：TFAR19단백재철제GM-CSF적TF-1세포중표체수평현저증고，반유명현적핵전위현상，차조우PS외번화세포DNA편단화。결론：TFAR19단백적핵전위시세포조망경조기발생적사건지일, 저일신발현위진일보탐토TFAR19단백적생물학효응，이급대세포조망적다방위연구제공료신적선색화사로。
Objective: To study the expression and localization of apoptosis-related protein TFAR19 in TF-1 cells undergoing apoptosis. Methods: Using monoclonal antibody against TFAR19, the expression level and cell localization of TFAR19 were examined by fluorescence microscope, confocal laser scan microscope(CLSM) and flow cytometry. Simultaneously, we also analyzed the relationship of TFAR19 protein with phosphatidylserine (PS) externalization and cell nuclear DNA fragmentation. Results: The level of TFAR19 proteins expressed in TF-1 cells treated with GM-CSF withdrawal was significantly increased compared with normal TF-1 cells, then translocated rapidly from cytoplasm to the nucleus of cells. Appearance of TFAR19 in the nucleus of apoptotic cells preceded the detection of PS externalization and DNA fragmentation. Conclusion: Nuclear translocation of TFAR19 protein is one of the earliest events of cell apoptotic process. These data provided a new clue to further approach to the biological function of TFAR19 and study of cell apoptosis.