CAJ | 학술논문

目的探讨 IL-6在 U937细胞中产生生物学效应的信号转导基础。方法检测 IL-6对 U937细胞生长与分化的影响，并采用凝胶阻滞电泳法，检测 IL-6在 U937细胞中激活核因子的情况。结果 IL-6可诱导 U937细胞向巨噬细胞方向分化，分化的细胞酸性醋酸酯酶 (ANAE)活性、硝基蓝四唑 (NBT)还原能力及 CD54表达增强。 STAT3可被 IL-6显著激活，其活性呈一定的剂量与时间依赖性；而 NF-IL6、 NF-κ B、 AP-1及其它 STAT家族成员则无明显激活。结论 U937细胞的终末分化可能是 JAK-STAT3通路介导的。
목적탐토 IL-6재 U937세포중산생생물학효응적신호전도기출。방법검측 IL-6대 U937세포생장여분화적영향，병채용응효조체전영법，검측 IL-6재 U937세포중격활핵인자적정황。결과 IL-6가유도 U937세포향거서세포방향분화，분화적세포산성작산지매 (ANAE)활성、초기람사서 (NBT)환원능력급 CD54표체증강。 STAT3가피 IL-6현저격활，기활성정일정적제량여시간의뢰성；이 NF-IL6、 NF-κ B、 AP-1급기타 STAT가족성원칙무명현격활。결론 U937세포적종말분화가능시 JAK-STAT3통로개도적。
Aim To investigate the signaling basis of the biological effects of IL-6 in U937 cells. Methods We determined the effect of IL-6 on the growth and differentiation of U937 cells and the effect of IL-6 on the activation of nuclear factors in U937 cells. Results IL-6 induced macrophage differentiation of U937 cells. Differentiated cells had strengthened ANAE (acid naphthyl acetate esterase)and NBT(nitroblue tetrazolium)-reducing activities and CD54 expression was upregulated. STAT3 could be remarkably activated by IL-6 in U937 cells and STAT3 activity was dependent on dose and time course of IL-6 treatment. However, NF-IL6,NF-κ B, AP-1 and other STAT members were not activated by IL-6. Conclusion IL-6 might induce terminal differentiation of U937 cells via JAK-STAT3 pathway.