CAJ | 학술논문

目的：研究脂多糖（LPS）作用下的血管平滑肌细胞血红素加氧酶-1(HO-1)基因的表达，并探讨HO/一氧化碳(CO)调节通路在内毒素性血管调节功能障碍中的作用。方法： 10 mg/L、30 mg/L及50 mg/L的LPS与培养的大鼠主动脉平滑肌细胞（VSMC）共同孵育6 h，以及10 mg/L LPS与VSMC共同孵育9 h和18 h。分别观察细胞丙二醛（MDA）含量及乳酸脱氢酶（LDH）活性、台盼蓝染色法记录VSMC蓝染率的变化；Northern杂交测HO-1 mRNA表达。结果： VSMC HO-1 mRNA表达随LPS浓度的增大而增加，LPS 50 mg/L时HO-1 mRNA表达比对照高176.7%；低剂量LPS（10 mg/L）诱导VSMC HO-1 mRNA的表达量随诱导时间的延长而增加，18 h组HO-1 mRNA的表达量比对照高195.6%。只有当LPS浓度增加至50 mg/L及10 mg/L孵育18 h时，细胞台盼蓝摄取率、MDA含量与LDH活性明显增加、细胞出现损伤。结论： LPS可诱导VSMC HO-1 mRNA的表达且具有浓度依赖性和时间依赖性，HO/CO可能为介导内毒素性血管平滑肌功能调节障碍的重要通路。
목적：연구지다당（LPS）작용하적혈관평활기세포혈홍소가양매-1(HO-1)기인적표체，병탐토HO/일양화탄(CO)조절통로재내독소성혈관조절공능장애중적작용。방법： 10 mg/L、30 mg/L급50 mg/L적LPS여배양적대서주동맥평활기세포（VSMC）공동부육6 h，이급10 mg/L LPS여VSMC공동부육9 h화18 h。분별관찰세포병이철（MDA）함량급유산탈경매（LDH）활성、태반람염색법기록VSMC람염솔적변화；Northern잡교측HO-1 mRNA표체。결과： VSMC HO-1 mRNA표체수LPS농도적증대이증가，LPS 50 mg/L시HO-1 mRNA표체비대조고176.7%；저제량LPS（10 mg/L）유도VSMC HO-1 mRNA적표체량수유도시간적연장이증가，18 h조HO-1 mRNA적표체량비대조고195.6%。지유당LPS농도증가지50 mg/L급10 mg/L부육18 h시，세포태반람섭취솔、MDA함량여LDH활성명현증가、세포출현손상。결론： LPS가유도VSMC HO-1 mRNA적표체차구유농도의뢰성화시간의뢰성，HO/CO가능위개도내독소성혈관평활기공능조절장애적중요통로。
AIM: To study the alterations of heme oxygenase-1 mRNA in vascular smooth muscle cells(VSMC) induced by lipopolysaccharide(LPS) and the role of heme oxygenase(HO)/carbon monoxide(CO)pathway in the disorders of regulation of cardiovascular system by LPS. METHODS: LPS (final concentrations 10 mg/L,30 mg/L and 50 mg/L) was added in cultured VSMCs for 6 h respectively or 10 mg/L LPS for 9 h and 18 h. MDA content, LDH release and the rate of trypan blue uptake of VSMC were measured. HO-1 mRNA expression was examined by Northern Blot. RESULTS: VSMC HO-1 mRNA expression was increased gradually with the increasing of LPS concentration. When final concentration of LPS was 50 mg/L, the HO-1 mRNA expression of VSMC was increased by 176.7% compared with control. When LPS final concentration was 10 mg/L, the HO-1 mRNA expression increased gradually along with the culture time. When cultured for 18 h, the HO-1 mRNA expression of VSMC was increased by 195.6% compared with control. Only at LPS 50 mg/L for 6 h and 10 mg/L for 18 h, the rate of trypan blue uptake，MDA content and LDH release were significantly increased. CONCLUSION: LPS can induce the HO-1 mRNA expression of VSMC and that were dose-dependent and time-dependent. The inducible HO may play an important role in the pathogenesis of vascular system under LPS.