CAJ | 학술논문

建立了应用毛细管胶束电动色谱（MECC）灵敏、快速的测定血管紧张素转化酶（ACE）活性的方法。通过对电压、上样时间、电极缓冲液体系等影响因素的优化，探讨了方法的可行性，确立了最佳测定条件(电压：8.1kV；上样时间：1s；电极缓冲液：20mmol/L硼酸盐缓冲液(pH9.0，含50mmol/LSDS)；检测波长：228nm)。方法的最低ACE活性检测限为5pmol/min(以2倍的信噪比计)。
건립료응용모세관효속전동색보（MECC）령민、쾌속적측정혈관긴장소전화매（ACE）활성적방법。통과대전압、상양시간、전겁완충액체계등영향인소적우화，탐토료방법적가행성，학립료최가측정조건(전압：8.1kV；상양시간：1s；전겁완충액：20mmol/L붕산염완충액(pH9.0，함50mmol/LSDS)；검측파장：228nm)。방법적최저ACE활성검측한위5pmol/min(이2배적신조비계)。
A sensitive and rapid method was developed for angiotensin-converting enzyme (ACE) activity determination by micellar electrokinetic capillary chromat ography (MECC). MECC was carried out to separate and quantify the products of t h e enzymatic reaction using Hip-Leu-His as the substrate in 20 mmol/L boric aci d- borate buffer (pH 9.0) including 50 mmol/L SDS as the run buffer at an applied vo ltage of 8.1 kV. The electrophoresis wa s m onitored at 228 nm, and completed in 6 minutes. The detection limits of ACE act ivity was 5 pmol/min(signal to noise ratio was 2).