CAJ | 학술논문

建立了同时测定化妆品中17种致敏原(茴香醇、苯甲醇、香豆素、肉桂醇、丁香酚、异丁香酚、香叶醇、芳樟醇、柠檬醛、2-辛炔酸甲酯、戊基肉桂醇、水杨酸苄酯、肉桂酸苯甲酯、戊基肉桂醛、α-异甲基紫罗兰酮、α-己基肉桂醛、苧烯)的高效液相色谱方法.不同类型的化妆品样品经无水乙醇超声提取,以 15 000 r/min离心15 min,取上清液过滤后测定.采用Agilent ZORBAX Eclipse XDB-C_8(4.6 mm×250 mm,5 μm)色谱柱,以乙腈-水为流动相梯度洗脱,流速1.0 mL/min,采用二极管阵列检测器多波长检测(210、230、250、275、300 nm).17种致敏原的定量下限为10 ～500 μg/kg,线性范围内相关系数均大于0.999.在高、中、低3个添加水平下的平均加标回收率(n=5)为85% ～118%,相对标准偏差为1.5% ～9.7%.该方法简便、快速、准确,适用于化妆品中17种致敏原的测定.
건립료동시측정화장품중17충치민원(회향순、분갑순、향두소、육계순、정향분、이정향분、향협순、방장순、저몽철、2-신결산갑지、무기육계순、수양산변지、육계산분갑지、무기육계철、α-이갑기자라란동、α-기기육계철、저희)적고효액상색보방법.불동류형적화장품양품경무수을순초성제취,이 15 000 r/min리심15 min,취상청액과려후측정.채용Agilent ZORBAX Eclipse XDB-C_8(4.6 mm×250 mm,5 μm)색보주,이을정-수위류동상제도세탈,류속1.0 mL/min,채용이겁관진렬검측기다파장검측(210、230、250、275、300 nm).17충치민원적정량하한위10 ～500 μg/kg,선성범위내상관계수균대우0.999.재고、중、저3개첨가수평하적평균가표회수솔(n=5)위85% ～118%,상대표준편차위1.5% ～9.7%.해방법간편、쾌속、준학,괄용우화장품중17충치민원적측정.
A high performance liquid chromatographic(HPLC) method was developed for the simultaneous determination of 17 allergens,including anise alcohol,benzyl alcohol,coumarin,cinnamyl alcohol,eugenol,isoeugenol,geraniol,linalool,citral,methylheptin carbonate,amylcinnamyl alcohol,benzyl salicylate,benzyl cinnamaldehyde,amyl cinnamal,α-isomethyl ionone,α-hexyl cinnamaldehyde and limonene in cosmetics.The cosmetic samples were extracted supersonically with anhydrous ethanol and centrifuged at 15 000 r/min for 15 min.The supernatant was separated on an Aglient Zorbax Eclipse XDB-C_8 column(4.6 mm × 250 mm,5 μm) by gradient elution with acetonitrile(A)-water(B) as the mobile phase at a flow rate of 1.0 mL/min.The analysis of 17 allergens was performed by HPLC with diode array detector at the wavelength of 210,230,250,275 and 300 nm,respectively.The limits of quantitation(LOQs) of 17 allergens were in the range of 10-500 μg/kg.The method showed a good linearity with correlation coefficients above 0.999.The mean recoveries at three spiked levels were in the range of 85%-118% with RSDs of 1.5%-9.7%.The method is accurate,rapid and suitable for the determination of 17 allergens in cosmetics sample.