CAJ | 학술논문

背景:研究表明部分肾移植受者在移植前就产生了MHC-I类链相关基因A(MICA)抗体,包括部分自身抗体,其急性排斥反应发生率明显高于抗体阴性者.目的:探讨外源性抗原对内皮细胞MICA表达的影响.方法:分别以5,10和25 μg/L 3个剂量,将MICA重组蛋白分为M5,M10,M25组,将热休克蛋白分为H5,H10,H25组,对人脐静脉内皮细胞予以诱导培养48 h,对照组加入等量的磷酸盐缓冲液.结果与结论:用MICA重组蛋白诱导48 h后,各实验组(M5,M10,M25)内皮细胞MICA mRNA和MICA蛋白的表达量与对照组比较均有显著增加(P < 0.05).M5组和M10组MICA mRNA和MICA蛋白均高于M25组 (P < 0.05).MICA膜蛋白表达以M10组最高,并与M5组、M25组之间差异有显著性意义(P < 0.05).各实验组(M5,M10,M25)可溶性MICA水平较对照组显著下降(P < 0.05).热休克蛋白组内皮细胞MICA基因的表达和可溶性MICA水平均无明显改变.结果表明外源性MICA抗原能够诱导内皮细胞自身MICA mRNA和蛋白表达上调,尤其是细胞膜蛋白增加明显,但可溶性MICA水平显著下降.
배경:연구표명부분신이식수자재이식전취산생료MHC-I류련상관기인A(MICA)항체,포괄부분자신항체,기급성배척반응발생솔명현고우항체음성자.목적:탐토외원성항원대내피세포MICA표체적영향.방법:분별이5,10화25 μg/L 3개제량,장MICA중조단백분위M5,M10,M25조,장열휴극단백분위H5,H10,H25조,대인제정맥내피세포여이유도배양48 h,대조조가입등량적린산염완충액.결과여결론:용MICA중조단백유도48 h후,각실험조(M5,M10,M25)내피세포MICA mRNA화MICA단백적표체량여대조조비교균유현저증가(P < 0.05).M5조화M10조MICA mRNA화MICA단백균고우M25조 (P < 0.05).MICA막단백표체이M10조최고,병여M5조、M25조지간차이유현저성의의(P < 0.05).각실험조(M5,M10,M25)가용성MICA수평교대조조현저하강(P < 0.05).열휴극단백조내피세포MICA기인적표체화가용성MICA수평균무명현개변.결과표명외원성MICA항원능구유도내피세포자신MICA mRNA화단백표체상조,우기시세포막단백증가명현,단가용성MICA수평현저하강.
BACKGROUND: Studies have demonstrated that incidence rate of acute rejection in renal transplant recipients with pre-production of major histocompatibility complex class I chain-related gene A (MICA), including parts of autoantibody, before transplantation in body, is obviously greater than that of recipients with negative antibody. OBJECTIVE: To investigate effects of exogenous antigen on MICA expression in endothelial cells. METHODS: The endothelial cells were cultured with exogenous recombinant MICA protein (group M5, M10 and M25) and heat shock protein-70 (group H5, H10 and H25) with dosages of 5, 10 and 25 μg/L, respectively, for 48 hours. Same volume of phosphate buffer saline was added into the control groups. RESULTS AND CONCLUSION: At 48 hours after induction, the expressions of MICA mRNA and protein were increased significantly in each experimental group (M5, M10 and M25) than that of the control group with significant (P < 0.05). The expression of MICA mRNA and MICA protein of group M5 and group M10 were remarkably higher than group M25 (P < 0.05); however, there was no significant difference between group M5 and M10 (P > 0.05). The expression of MICA membrane protein in the group M10 was obviously greater than that of the group M5 and M25 (P < 0.05). The level of soluble MICA (sMICA) in experimental groups (M5, M10 and M25) was decreased obviously comparing with that of the control group. These differences had statistical significances (P < 0.05). But there was no significant difference among the experimental groups (P > 0.05). However, the expression of MICA gene and sMICA level did not change after heat shock protein-70 stimulation. The exogenous MICA antigen up-regulates the expression of MICA mRNA and protein, especially increases the expression of membrane protein on the cell surface significantly, but sMICA in supernatant was dramatically decreased.