CAJ | 학술논문

目的对HIV-1感染患儿的人类白细胞抗原(HLA)-Ⅰ基因型进行分析,探讨其对HIV-1特异性CTL应答的影响.方法采用酶联免疫斑点技术(ELISPOT),以HIV-1 P24区域的氨基酸序列人工合成的12个重叠肽段组成的肽段库作为特异性抗原表位,对5例接受HAART后的HIV-1感染患儿外周血单核细胞(PBMC)的IFN-γ分泌细胞频数进行测定研究.同时,还采用了PCR-序列特异引物技术(PCR-SSP)及PCR-直接碱基序列分析基因分型技术(PCR-SBT)对这5例患儿进行了HLA-Ⅰ等位基因分型及HLA-B*高分辨等位基因分型.结果 5例HIV-1感染息儿中,4例HLA-B*基因型为B*40,其中3例为HLA-B*4001,1例为HLA-B*4002.其HIV-1抗原特异性CTL应答水平差异明显.5例患儿均未出现免疫重建炎症综合征.结论基因型为HLA-B*40的儿童可能更易感染HIV-1,HLA-B*40可能影响HIV-1感染患儿的抗原特异性CTL应答.
목적 대HIV-1감염환인적인류백세포항원(HLA)-Ⅰ기인형진행분석,탐토기대HIV-1특이성CTL응답적영향.방법 채용매련면역반점기술(ELISPOT),이HIV-1 P24구역적안기산서렬인공합성적12개중첩태단조성적태단고작위특이성항원표위,대5례접수HAART후적HIV-1감염환인외주혈단핵세포(PBMC)적IFN-γ분비세포빈수진행측정연구.동시,환채용료PCR-서렬특이인물기술(PCR-SSP)급PCR-직접감기서렬분석기인분형기술(PCR-SBT)대저5례환인진행료HLA-Ⅰ등위기인분형급HLA-B*고분변등위기인분형.결과 5례HIV-1감염식인중,4례HLA-B*기인형위B*40,기중3례위HLA-B*4001,1례위HLA-B*4002.기HIV-1항원특이성CTL응답수평차이명현.5례환인균미출현면역중건염증종합정.결론 기인형위HLA-B*40적인동가능경역감염HIV-1,HLA-B*40가능영향HIV-1감염환인적항원특이성CTL응답.
Objective To analyze human leucocyte antigen-Ⅰ (HLA-Ⅰ)genotype of HIV-1-infected-children and explore the influence of HLA-Ⅰ on HIV-1 specific cytotoxic T lymphocyte (CTL)responses. Methods Frequency of IFN-γ secreting cells in PBMC from 5 HAART-treated HIV-1-infected children were assessed by stimulation with a peptide pool containing 12 overlapping peptdes in HIV-1 P24, by using assay of enzyme-linked immunospot (ELISPOT).Moreover, HLA-Ⅰ alleles of them were genotyped by assays of polymerase chain reaction-sequence specific primer (PCRSSP) and high-resolution subtyping using PCR-sequence based genotyping (PCR-SBT). Results Among 5 HIV-1infected-children, there were 4 cases with HLA-B* genotype, including 3 cases with HLA-B* 4001 and 1 case with HLA-B* 4002. Frequency of HIV-1 antigen specific CTL responses existed obvious differences. None of 5 HIV-1 infected-children developed immune reconstitution inflammtory syndrome. Conclusions Children with HLA-B* 40genotype may be susceptible to HIV-1. HLA-B* 40 may affect HIV-1 specific CTL responses in HIV-1-infected children.