中华精神科杂志
中華精神科雜誌
중화정신과잡지
CHINESE JOURNA OF PSYCHIATRY
2008年
4期
237-240
,共4页
李卫东%吴元坪%韩止荣%戴巍%马青%潘可欣%图娅
李衛東%吳元坪%韓止榮%戴巍%馬青%潘可訢%圖婭
리위동%오원평%한지영%대외%마청%반가흔%도아
应激%抑郁症%电针%氟西汀%行为%海马%细胞凋亡
應激%抑鬱癥%電針%氟西汀%行為%海馬%細胞凋亡
응격%억욱증%전침%불서정%행위%해마%세포조망
Stress%Depression%Electroacupuneture%Fluoxetine%Behavior%Hippocampus%Apoptosis
目的 探究电针及氟西汀对慢性应激抑郁大鼠行为学及海马神经元凋亡的影响.方法 65只Sprague-Dawley雄性大鼠,按随机数字表随机分为空白组、空白电针组、模型组、电针组、氟西汀组,每组13只.慢性应激后进行行为学评价;采用膜联蛋白V-异硫氰酸荧光素/碘化丙啶双染法检测海马神经元凋亡率;采用免疫组织化学方法检测海马bcl-2蛋白表达.结果 模型组大鼠旷场试验水平穿越格数[(1.6±1.3)格]、竖立次数[(0.4 ±0.2)次]、体质量增加量[(34±18)g]均明显低于空白组[分别为(51.1 ±22.3)格、(13.2±4.6)次、(128 ±21)g],P均＜0.05;海马神经元细胞凋亡率[(67±10)%]高于空白组[(53±13)%],P＜0.05;海马组织bcl-2蛋白阳性细胞计数[(28±10)个/mm<'2>]低于空白组[(78±22)个/mm<'2>],P＜0.05.电针组[分别为(39.3 ±14.3)格,(9.6 ±4.1)次,(81±43)g]、氟西汀组[分别为(37.2±15.1)格,(9.3±4.6)次,(80 ±35)g]上述指标均明显高于模型组(P＜0.05);海马神经元细胞凋亡率[电针组(30±9)%,氟西汀组(51±13)%]均低于模型组(P＜0.05);海马组织bcl-2蛋白阳性细胞计数[电针组(56±18)个/mm<'2>,氟西汀组(62±24)个/mm<'2>]均高于模型组(P＜0.05),而电针组与氟西汀组间的差异无统计学意义(P＞0.05).结论 电针和氟西汀可改善抑郁大鼠行为学症状,这种改善与海马细胞凋亡机制有一定相关性.
目的 探究電針及氟西汀對慢性應激抑鬱大鼠行為學及海馬神經元凋亡的影響.方法 65隻Sprague-Dawley雄性大鼠,按隨機數字錶隨機分為空白組、空白電針組、模型組、電針組、氟西汀組,每組13隻.慢性應激後進行行為學評價;採用膜聯蛋白V-異硫氰痠熒光素/碘化丙啶雙染法檢測海馬神經元凋亡率;採用免疫組織化學方法檢測海馬bcl-2蛋白錶達.結果 模型組大鼠曠場試驗水平穿越格數[(1.6±1.3)格]、豎立次數[(0.4 ±0.2)次]、體質量增加量[(34±18)g]均明顯低于空白組[分彆為(51.1 ±22.3)格、(13.2±4.6)次、(128 ±21)g],P均＜0.05;海馬神經元細胞凋亡率[(67±10)%]高于空白組[(53±13)%],P＜0.05;海馬組織bcl-2蛋白暘性細胞計數[(28±10)箇/mm<'2>]低于空白組[(78±22)箇/mm<'2>],P＜0.05.電針組[分彆為(39.3 ±14.3)格,(9.6 ±4.1)次,(81±43)g]、氟西汀組[分彆為(37.2±15.1)格,(9.3±4.6)次,(80 ±35)g]上述指標均明顯高于模型組(P＜0.05);海馬神經元細胞凋亡率[電針組(30±9)%,氟西汀組(51±13)%]均低于模型組(P＜0.05);海馬組織bcl-2蛋白暘性細胞計數[電針組(56±18)箇/mm<'2>,氟西汀組(62±24)箇/mm<'2>]均高于模型組(P＜0.05),而電針組與氟西汀組間的差異無統計學意義(P＞0.05).結論 電針和氟西汀可改善抑鬱大鼠行為學癥狀,這種改善與海馬細胞凋亡機製有一定相關性.
목적 탐구전침급불서정대만성응격억욱대서행위학급해마신경원조망적영향.방법 65지Sprague-Dawley웅성대서,안수궤수자표수궤분위공백조、공백전침조、모형조、전침조、불서정조,매조13지.만성응격후진행행위학평개;채용막련단백V-이류청산형광소/전화병정쌍염법검측해마신경원조망솔;채용면역조직화학방법검측해마bcl-2단백표체.결과 모형조대서광장시험수평천월격수[(1.6±1.3)격]、수립차수[(0.4 ±0.2)차]、체질량증가량[(34±18)g]균명현저우공백조[분별위(51.1 ±22.3)격、(13.2±4.6)차、(128 ±21)g],P균＜0.05;해마신경원세포조망솔[(67±10)%]고우공백조[(53±13)%],P＜0.05;해마조직bcl-2단백양성세포계수[(28±10)개/mm<'2>]저우공백조[(78±22)개/mm<'2>],P＜0.05.전침조[분별위(39.3 ±14.3)격,(9.6 ±4.1)차,(81±43)g]、불서정조[분별위(37.2±15.1)격,(9.3±4.6)차,(80 ±35)g]상술지표균명현고우모형조(P＜0.05);해마신경원세포조망솔[전침조(30±9)%,불서정조(51±13)%]균저우모형조(P＜0.05);해마조직bcl-2단백양성세포계수[전침조(56±18)개/mm<'2>,불서정조(62±24)개/mm<'2>]균고우모형조(P＜0.05),이전침조여불서정조간적차이무통계학의의(P＞0.05).결론 전침화불서정가개선억욱대서행위학증상,저충개선여해마세포조망궤제유일정상관성.
Objective To study the effects of electroacupuncture and fluoxetine on apoptosis in the hippocampus in chronic stress depression rats.Methods Sixty-five Sprague-Dawley male rats were randomly divided into control group,control group combining with electroacupuncture,model group, electroacupuncture group and fluoxetine group.The model was produced by chronic unpredictable mild stress.Open-filed test was used to detect the behavior of rats.Annexin V-FITC/ PI method was used to detect the apoptotic rates in the hippecampus.Immunohistocbemical technique was used to detect the expression of bcl-2 protein.Results After stress,the model group rats' crossing numbers ( 1.6 ± 1.3 ), rearing times (0.4 ±0.2),body weight changes(34 ± 18) g were markedly less than that of control group rats'[(51.1 ±22.3)times,(13.2 ±4.6)times,( 128 ±21 )g;P ＜0.05],and the hippocampal apoptotic rates and the expression of bcl-2 was higher in model group than control group ( P ＜ 0.05 ).In comparison with the model group,the crossing times,rearing times,body weight changes increased in electroacupuncture group[(39.3 ±14.3 ) times,( 9.6 ±4.1 ) times,( 81 ±43 ) g]and fluoxetine group[(37.2 ± 15.1 ) times,(9.3 ± 4.6) times,( 80 ± 35 ) g],( P ＜ 0.05 ),and the apoptotic rates of the electroaxupuncture group and fluoxetine group were lower ( P ＜ 0.05 ),the expression of bcl-2 were higher ( P ＜ 0.05 ).Those indexes have not apparently differences between electroacupuncture group and fluoxetine group ( P＞ 0.05).Conclusion Electroacupuncture and fluoxetine treatment could improve the behavior of depression induced by stress,which may be related to hippecampal apoptosis.
