国际医学寄生虫病杂志
國際醫學寄生蟲病雜誌
국제의학기생충병잡지
INTERNATIONAL JOURNAL OF MEDICAL PARASITIC DISEASES
2010年
3期
132-135
,共4页
李云华%朱诗应%孙庆文%朱淮民
李雲華%硃詩應%孫慶文%硃淮民
리운화%주시응%손경문%주회민
疟原虫%热激蛋白%差异表达基因%全长扩增
瘧原蟲%熱激蛋白%差異錶達基因%全長擴增
학원충%열격단백%차이표체기인%전장확증
Plasmodium%Heat shock protein%Differentially expressed genes%RACE amplification
目的 为获取中华按蚊感染约氏疟原虫后24 h的高表达基因热激蛋白40(HSP40)cDNA的全长序列. 方法 运用抑制性消减杂交技术(suppression substractive hybridization,SSH)构建中华按蚊感染约氏疟原虫后24 h的差异表达基因文库,从高表达文库中获得长度为363 bp的HSP40基因的cDNA片段,运用RACE技术对其cDNA序列进行全长扩增. 结果 获得中华按蚊HSP40 cDNA的全长序列,总长度为1 159 bp,开放阅读框为1 014 bp,编码337个氨基酸. 结论 获得了中华按蚊HSP40 cDNA的全长序列.
目的 為穫取中華按蚊感染約氏瘧原蟲後24 h的高錶達基因熱激蛋白40(HSP40)cDNA的全長序列. 方法 運用抑製性消減雜交技術(suppression substractive hybridization,SSH)構建中華按蚊感染約氏瘧原蟲後24 h的差異錶達基因文庫,從高錶達文庫中穫得長度為363 bp的HSP40基因的cDNA片段,運用RACE技術對其cDNA序列進行全長擴增. 結果 穫得中華按蚊HSP40 cDNA的全長序列,總長度為1 159 bp,開放閱讀框為1 014 bp,編碼337箇氨基痠. 結論 穫得瞭中華按蚊HSP40 cDNA的全長序列.
목적 위획취중화안문감염약씨학원충후24 h적고표체기인열격단백40(HSP40)cDNA적전장서렬. 방법 운용억제성소감잡교기술(suppression substractive hybridization,SSH)구건중화안문감염약씨학원충후24 h적차이표체기인문고,종고표체문고중획득장도위363 bp적HSP40기인적cDNA편단,운용RACE기술대기cDNA서렬진행전장확증. 결과 획득중화안문HSP40 cDNA적전장서렬,총장도위1 159 bp,개방열독광위1 014 bp,편마337개안기산. 결론 획득료중화안문HSP40 cDNA적전장서렬.
Objective To isolate differentially expressed gene HSP40 from Anopheles sinensis induced by infection with Plasmodium yoelii yoelii after 24 h, and to amplify the full-length cDNA of HSP40. Methods A 363 bp of HSP40 cDNA was isolated from the suppression substractive hybridization (SSH) library. The full length of cDNA was amplified using the RACE (rapid-amplification of cDNA ends) technology. Results The full-length cDNA of HSP40 was completely amplified. The whole length was 1 159 bp, with an open reading frame of 1 014 bp, encoding 337 amino acids. Conclusions The full-length cDNA of HSP40 has been completely amplified.
