中华行为医学与脑科学杂志
中華行為醫學與腦科學雜誌
중화행위의학여뇌과학잡지
CHINESE JOURNAL OF BEHAVIORAL MEDICINE AND BRAIN SCIENCE
2012年
6期
505-507
,共3页
海马%多巴胺%恐惧记忆
海馬%多巴胺%恐懼記憶
해마%다파알%공구기억
Hippocampal%Dopamine%Fear memory
目的 研究大鼠海马CA3区多巴胺(DA)能系统在条件性恐惧记忆形成与保持中的作用.方法 条件性恐惧训练前2周向双侧海马CA3区注入6-羟基多巴胺(6-OHDA)进行损毁,训练后用Western blotting检测前额叶皮层、CA1、杏仁体GluR1及NR2B的表达变化.结果 (1)与生理盐水组[(66.44±16.58)％,(73.43±23.57)％,(55.27±20.57)％]比较,6-OHDA损毁组恐惧记忆获得的僵住反应[ (65.58±5.33)％]差异无统计学意义(P＞0.05),短时恐惧记忆的僵住反应[(39.24±12.83)％]与长时恐惧记忆的僵住反应[(31.15±6.51)％]明显减少(P＜0.05).(2)与生理盐水组比较,6-OHDA损毁组大鼠前额叶皮层,海马CA1区的GluR1蛋白表达差异无统计学意义(P＞0.05),杏仁体BLA区的GluR1蛋白表达升高(P＜0.01).与生理盐水组比较,6-OHDA损毁组大鼠前额叶皮层的NR2B蛋白表达升高(P＜0.01),海马CA1区的NR2B蛋白表达差异无统计学意义(P＞0.05),杏仁体BLA区的NR2B蛋白表达降低(P＜0.01).结论 大鼠海马CA3区多巴胺能系统功能下调能损害恐惧记忆的巩固但不影响其获得,还可以调节其他脑区记忆相关蛋白的表达.
目的 研究大鼠海馬CA3區多巴胺(DA)能繫統在條件性恐懼記憶形成與保持中的作用.方法 條件性恐懼訓練前2週嚮雙側海馬CA3區註入6-羥基多巴胺(6-OHDA)進行損燬,訓練後用Western blotting檢測前額葉皮層、CA1、杏仁體GluR1及NR2B的錶達變化.結果 (1)與生理鹽水組[(66.44±16.58)％,(73.43±23.57)％,(55.27±20.57)％]比較,6-OHDA損燬組恐懼記憶穫得的僵住反應[ (65.58±5.33)％]差異無統計學意義(P＞0.05),短時恐懼記憶的僵住反應[(39.24±12.83)％]與長時恐懼記憶的僵住反應[(31.15±6.51)％]明顯減少(P＜0.05).(2)與生理鹽水組比較,6-OHDA損燬組大鼠前額葉皮層,海馬CA1區的GluR1蛋白錶達差異無統計學意義(P＞0.05),杏仁體BLA區的GluR1蛋白錶達升高(P＜0.01).與生理鹽水組比較,6-OHDA損燬組大鼠前額葉皮層的NR2B蛋白錶達升高(P＜0.01),海馬CA1區的NR2B蛋白錶達差異無統計學意義(P＞0.05),杏仁體BLA區的NR2B蛋白錶達降低(P＜0.01).結論 大鼠海馬CA3區多巴胺能繫統功能下調能損害恐懼記憶的鞏固但不影響其穫得,還可以調節其他腦區記憶相關蛋白的錶達.
목적 연구대서해마CA3구다파알(DA)능계통재조건성공구기억형성여보지중적작용.방법 조건성공구훈련전2주향쌍측해마CA3구주입6-간기다파알(6-OHDA)진행손훼,훈련후용Western blotting검측전액협피층、CA1、행인체GluR1급NR2B적표체변화.결과 (1)여생리염수조[(66.44±16.58)％,(73.43±23.57)％,(55.27±20.57)％]비교,6-OHDA손훼조공구기억획득적강주반응[ (65.58±5.33)％]차이무통계학의의(P＞0.05),단시공구기억적강주반응[(39.24±12.83)％]여장시공구기억적강주반응[(31.15±6.51)％]명현감소(P＜0.05).(2)여생리염수조비교,6-OHDA손훼조대서전액협피층,해마CA1구적GluR1단백표체차이무통계학의의(P＞0.05),행인체BLA구적GluR1단백표체승고(P＜0.01).여생리염수조비교,6-OHDA손훼조대서전액협피층적NR2B단백표체승고(P＜0.01),해마CA1구적NR2B단백표체차이무통계학의의(P＞0.05),행인체BLA구적NR2B단백표체강저(P＜0.01).결론 대서해마CA3구다파알능계통공능하조능손해공구기억적공고단불영향기획득,환가이조절기타뇌구기억상관단백적표체.
Objective To investigate the effects of hippocampal CA3 dopaminergic system in acquisition and consolidation of Pavlovian fear conditioning,and expression of GluR1 and NR2B in medial prefrontal cortex (mPFC),CA1 and basolateral amygdala (BLA) after fear conditioning training.Methods Bilateral injection 6-OHDA into hippocampal CA3 to lesion dopaminergic fibers 2 weeks before fear conditioning training.The change of GluR1 and NR2B were analyzed by western blot after training.Results Compared with the saline group ( (66.44 ± 16.58)％ ),there were significant decreases ( (39.24 ± 12.83)％,(31.15 ±6.51 )％ ) in the consolidation of short- and long- term fear memory (P ＜ 0.05 ) but not the acquisition ( ( 65.58 ± 5.33 ) ％,P ＞ 0.05).The expression of GluR1 protein was significantly increased in BLA (P＜0.01 ) but not the mPFC or hippocampal CA1 (P＞1.05 ),compared with the saline group.In addition,the expression of NR2B protein was significantly increased in the mPFC and decreased in BLA (P＜0.01) but not the hippocampal CA1 (P＞0.05),compared with the saline group.Conclusion Down-regulation of dopaminergic system in hippocampal CA3 may impair the consolidation but not the acquisition of fear memory,and also regulate the expression of GluR1,NR2B protein related to fear memory in other brain regions.
