CAJ | 학술논문

目的分析中国各地HIV-1感染者的vpr基因多态性,比较HIV-1 vpr基因变异的基本特征及其与国外以往研究的异同,为进一步探索HIV-1 vpr的致病机理及可能的基因治疗靶位打下基础.方法提取348例中国各地区HIV-1感染者血浆病毒RNA,运用逆转录聚合酶链式反应(RT-PCR)扩增HIV-1 vpr基因,凝胶电泳鉴定后行基因测序.用Blast、Clustal W、Mega4.0等相关生物分析软件进行vPr基因核苷酸和vpr氨基酸序列分析,并构建进化树,分析中国HIV-1感染者的vpr基因的多态性、变异特征和亚型的分子流行病学特征.结果按vpr基因分型共发现AB、AE、BC、B、C 5种HIV-1亚型,分别占2.60%,55.19%,2.60%,18.18%,21.43%,其中AE亚型比例最大.观察到vpr氨基酸序列有9个变异率大于20%的位点.结论首次分析中国感染者的HIV-1vpr基因多态性及其基因进化树的特征,发现一些vpr变异率较大的氨基酸位点,这些Vpr氨基酸序列的变异与其致病能力的关系有待进一步研究.
목적 분석중국각지HIV-1감염자적vpr기인다태성,비교HIV-1 vpr기인변이적기본특정급기여국외이왕연구적이동,위진일보탐색HIV-1 vpr적치병궤리급가능적기인치료파위타하기출.방법 제취348례중국각지구HIV-1감염자혈장병독RNA,운용역전록취합매련식반응(RT-PCR)확증HIV-1 vpr기인,응효전영감정후행기인측서.용Blast、Clustal W、Mega4.0등상관생물분석연건진행vPr기인핵감산화vpr안기산서렬분석,병구건진화수,분석중국HIV-1감염자적vpr기인적다태성、변이특정화아형적분자류행병학특정.결과 안vpr기인분형공발현AB、AE、BC、B、C 5충HIV-1아형,분별점2.60%,55.19%,2.60%,18.18%,21.43%,기중AE아형비례최대.관찰도vpr안기산서렬유9개변이솔대우20%적위점.결론 수차분석중국감염자적HIV-1vpr기인다태성급기기인진화수적특정,발현일사vpr변이솔교대적안기산위점,저사Vpr안기산서렬적변이여기치병능력적관계유대진일보연구.
Objective To detect and analyze vpr gene diversity of human immunodeficiency virus type 1(HIV-1)from patients with HIV in China.Different of mutated sites and molecular epidemic charac-tefisfics between vpr gene in China and abroad were compared to explore pathogenesis mechanisms of HIV-1 and possible gene therapy sites in the future.Methods Reverse transcription polymerase chain reaction uals.BLAST,Clustal W and Mega biological analysis software were applied to analyze sequences,and identify the characteristics of HIV-1 vpr gene polymorphism and its clinical significance in China.The uproo-ted tree was calculated according to the neighbor-joining method.Results HIV-1 vpr subtypes in China were CRFO1-AE(51.19%),subtypes C(21.43%),subtype B(18.18%),CRFO3-AB(2.60%)and CRFO8-BC(2.60%).Nine sites in vpr deduced amino acid sequences showed more than 20%mutated ra-tio.Conclusion This paper firsdy analyzed HIV-1 vpr gene diversity and the characteristics of vpr gene tree from Chinese patients.Some sites in vpr deduced amino acid sequences showed obvious mutated ratio,which were possibly correlated with clinical manifestation of HIV-1 infected individuals.