CAJ | 학술논문

目的检测CIDEA基因在脂肪瘤间充质干细胞中的表达,并探讨其与脂肪组织肿瘤的关系.方法采用实时荧光定量逆转录-聚合酶链反应(RT-PCR)检测6例脂肪瘤间充质干细胞和6例正常脂肪组织来源干细胞中CIDEA的表达水平.结果 CIDEA在脂肪瘤间充质干细胞中的表达显著低于正常脂肪来源干细胞,提示其在脂肪瘤间充质干细胞中表达的下降或缺失可能与肿瘤干细胞引发脂肪组织肿瘤相关.结论 CIDEA与脂肪细胞的分化呈现相关性,其表达的下降或缺失可能与脂肪组织肿瘤的发生发展有关.
목적 검측CIDEA기인재지방류간충질간세포중적표체,병탐토기여지방조직종류적관계.방법 채용실시형광정량역전록-취합매련반응(RT-PCR)검측6례지방류간충질간세포화6례정상지방조직래원간세포중CIDEA적표체수평.결과 CIDEA재지방류간충질간세포중적표체현저저우정상지방래원간세포,제시기재지방류간충질간세포중표체적하강혹결실가능여종류간세포인발지방조직종류상관.결론 CIDEA여지방세포적분화정현상관성,기표체적하강혹결실가능여지방조직종류적발생발전유관.
Objective To explore the expression level of CIDEA in lipoma mesenchymal stem cells (LMSCs) and the relationship between CIDEA expression and lipomas. Methods Real-time fluorescence quota RT-PCR was used to examine the CIDEA expression level in 6 cases of lipoma mesenchymal stem cells and 6 cases of adipose tissue-derived stem cells (ASCs). Results The expression of CIDEA in LMSCs was significantly lower than in ASCs. Conclusion CIDEA presents relevance with adipose cell differentiation.And its decline or loss is probably associated with the occurrence of tumors in adipose tissue.