中华实验和临床病毒学杂志
中華實驗和臨床病毒學雜誌
중화실험화림상병독학잡지
CHINESE JOURNAL OF EXPERIMENTAL AND CLINICAL VIROLOGY
2011年
5期
351-354
,共4页
尤凤兴%孙纳%马广源%王苗苗%沙丹%施超%吴家林%凌霞%肖勇%钱燕华%姜永%谢洁%季亚勇%管红霞
尤鳳興%孫納%馬廣源%王苗苗%沙丹%施超%吳傢林%凌霞%肖勇%錢燕華%薑永%謝潔%季亞勇%管紅霞
우봉흥%손납%마엄원%왕묘묘%사단%시초%오가림%릉하%초용%전연화%강영%사길%계아용%관홍하
流感病毒A型%中和试验
流感病毒A型%中和試驗
류감병독A형%중화시험
Influenza A virus%Neutralization tests
目的 调查无锡市人群中甲型、乙型流感病毒抗体水平和新甲型H1N1流感病毒传入前后人群中抗体水平,并对新甲型H1N1流感病毒传入1年后自然人群中成人抗体水平与接种新甲型H1N1流感疫苗后1年的成人抗体水平进行比较.方法 收集2008年9月至2009年5月、2010年9月至2011年1月无锡市不同年龄段人群血清和接种新甲型H1N1流感疫苗1年的成人血清,用血凝抑制(HI)试验测定抗体,并比较不同时间段各人群中的流感抗体阳性率、保护率和几何平均滴度(GMT).结果 新甲型H1N1流感病毒传入前,无锡市自然人群的HI抗体阳性率为2.86％ (4/140),保护率为0.71％(1/140),GMT为5.23.新甲型H1N1流感病毒传入1年后,自然人群的HI抗体阳性率为66.33％,保护率为37.76％、GMT为19.17;其中成人HI抗体阳性率、保护率和GMT分别为50.00％、19.44％和13.09.接种新甲型H1N1流感疫苗的成人1年后HI抗体阳性率、保护率和GMT分别为61.36％、22.73％和14.14,与自然人群中成人在流感病毒传入1年后的抗体水平差异无统计学意义(P均＞0.05).无锡市人群中甲型与乙型流感病毒HI抗体水平分别为:H1N1病毒抗体阳性率为55.00％,保护率为35.00％,GMT16.90;H3N2抗体阳性率为86.40％,保护率为84.30％,GMT为58.56.结论 新甲型H1N1流感病毒传入无锡市1年后,自然人群中新甲型H1N1流感病毒抗体阳性率、保护率和GMT均已达到季节性流感抗体水平.同时人群中已有一定水平的甲型、乙型流感病毒抗体,近期不会发生较大的季节性流感疫情.
目的 調查無錫市人群中甲型、乙型流感病毒抗體水平和新甲型H1N1流感病毒傳入前後人群中抗體水平,併對新甲型H1N1流感病毒傳入1年後自然人群中成人抗體水平與接種新甲型H1N1流感疫苗後1年的成人抗體水平進行比較.方法 收集2008年9月至2009年5月、2010年9月至2011年1月無錫市不同年齡段人群血清和接種新甲型H1N1流感疫苗1年的成人血清,用血凝抑製(HI)試驗測定抗體,併比較不同時間段各人群中的流感抗體暘性率、保護率和幾何平均滴度(GMT).結果 新甲型H1N1流感病毒傳入前,無錫市自然人群的HI抗體暘性率為2.86％ (4/140),保護率為0.71％(1/140),GMT為5.23.新甲型H1N1流感病毒傳入1年後,自然人群的HI抗體暘性率為66.33％,保護率為37.76％、GMT為19.17;其中成人HI抗體暘性率、保護率和GMT分彆為50.00％、19.44％和13.09.接種新甲型H1N1流感疫苗的成人1年後HI抗體暘性率、保護率和GMT分彆為61.36％、22.73％和14.14,與自然人群中成人在流感病毒傳入1年後的抗體水平差異無統計學意義(P均＞0.05).無錫市人群中甲型與乙型流感病毒HI抗體水平分彆為:H1N1病毒抗體暘性率為55.00％,保護率為35.00％,GMT16.90;H3N2抗體暘性率為86.40％,保護率為84.30％,GMT為58.56.結論 新甲型H1N1流感病毒傳入無錫市1年後,自然人群中新甲型H1N1流感病毒抗體暘性率、保護率和GMT均已達到季節性流感抗體水平.同時人群中已有一定水平的甲型、乙型流感病毒抗體,近期不會髮生較大的季節性流感疫情.
목적 조사무석시인군중갑형、을형류감병독항체수평화신갑형H1N1류감병독전입전후인군중항체수평,병대신갑형H1N1류감병독전입1년후자연인군중성인항체수평여접충신갑형H1N1류감역묘후1년적성인항체수평진행비교.방법 수집2008년9월지2009년5월、2010년9월지2011년1월무석시불동년령단인군혈청화접충신갑형H1N1류감역묘1년적성인혈청,용혈응억제(HI)시험측정항체,병비교불동시간단각인군중적류감항체양성솔、보호솔화궤하평균적도(GMT).결과 신갑형H1N1류감병독전입전,무석시자연인군적HI항체양성솔위2.86％ (4/140),보호솔위0.71％(1/140),GMT위5.23.신갑형H1N1류감병독전입1년후,자연인군적HI항체양성솔위66.33％,보호솔위37.76％、GMT위19.17;기중성인HI항체양성솔、보호솔화GMT분별위50.00％、19.44％화13.09.접충신갑형H1N1류감역묘적성인1년후HI항체양성솔、보호솔화GMT분별위61.36％、22.73％화14.14,여자연인군중성인재류감병독전입1년후적항체수평차이무통계학의의(P균＞0.05).무석시인군중갑형여을형류감병독HI항체수평분별위:H1N1병독항체양성솔위55.00％,보호솔위35.00％,GMT16.90;H3N2항체양성솔위86.40％,보호솔위84.30％,GMT위58.56.결론 신갑형H1N1류감병독전입무석시1년후,자연인군중신갑형H1N1류감병독항체양성솔、보호솔화GMT균이체도계절성류감항체수평.동시인군중이유일정수평적갑형、을형류감병독항체,근기불회발생교대적계절성류감역정.
Objective To investigate levels of antibodies against type A and type C influenza viruses and those against the 2009 H1N1 influenza A virus (before and after the 2009 H1N1 pandemic )among residents in Wuxi.To compare levels of antibodies against the 2009 H1N1 influenza virus (one year after the pandemic) in the unvaccinated population with those in the population who received vaccine.Methods Serum samples were collected from subjects (aged 1-60 years) during September 2008 to May 2009,and during September 2010 to January 2011.Also collected were serum samples from adults who had received vaccines for pandemic (H1N1) 2009 for one year.Antibody response to influenza viruses was measured using hemagglutination inhibition (HI) assay.Seropositivity rate,seroprotection rate and geometric mean titer (GMT) were compared for each age group during different periods.Results Before the outbreak of the 2009 H1N1 pandemic,seropositivity rate,seroprotection rate and GMT among the study subjects in were 2.86％ (4/140),0.71％ (1/140) and 5.23,respectively.One year after the outbreak,seropositivity rate,seroprotection rate and GMT among the study subjects were 66.33％,37.76％ and 19.17,respectively.Among them,adult subjects showed 50.00％ seropositivity rate,19.44％seroprotection rate and 13.09 GMT,while adult subjects who had received vaccine for one year showed 61.36％ seropositivity rate,22.73％ seroprotection rate and 14.14 GMT.No significant difference was observed between these two populations (P ＞ 0.05 for all three indexes).Furthermore,before the outbreak of the 2009 H1N1 pandemic,levels of antibodies against seasonal influenza viruses among the study subjects were as follows:for H1N1 virus,seropositivity rate,seroprotection rate and GMT were 55.00％,35.00％and 16.90,respectively; for H3N2 virus,seropositivity rate,seroprotection rate and GMT were 86.40％,84.30％ and 58.56,respectively.Conclusion One year after the 2009 H1N1 influenza A virus had spread to Wuxi,the population levels of antibodies against this virus have approached those against seasonal influenza viruses,as reflected by seropositivity rates,seroproection rates and GMT.Moreover,considerable levels of antibodies against seasonal influenza viruses were observed in populations,indicating no seasonal influenza outbreak would occur recently.
