CAJ | 학술논문

目的建立一种能更有效检测单拷贝基因的引物原位标记技术(primed in situ labeling,PRINS).方法在传统PRINS技术基础上,引入TsqStatrt抗体、Y染色体上的性别决定区基因(SIBX determining region Y,SRY)4条引物和TSATM Biotin System来检测SRY基因,并以对SRY基因的荧光原位杂交(fluorescence in situ hybridization,FISH)为对照.结果在PRINS结果中,通过对50个中期分裂相的分析,在Yp11.3的位置上都枪测到特异信号且清晰可辨别,此结果与FISH结果一致且标记信号强度相当.结论这项改进后的PRINS技术可快速针对单拷贝基因和小DNA片段进行检测.因此,相对于昂贵且费时的FISH技术,是另一种有效的选择.
목적 건립일충능경유효검측단고패기인적인물원위표기기술(primed in situ labeling,PRINS).방법 재전통PRINS기술기출상,인입TsqStatrt항체、Y염색체상적성별결정구기인(SIBX determining region Y,SRY)4조인물화TSATM Biotin System래검측SRY기인,병이대SRY기인적형광원위잡교(fluorescence in situ hybridization,FISH)위대조.결과 재PRINS결과중,통과대50개중기분렬상적분석,재Yp11.3적위치상도창측도특이신호차청석가변별,차결과여FISH결과일치차표기신호강도상당.결론 저항개진후적PRINS기술가쾌속침대단고패기인화소DNA편단진행검측.인차,상대우앙귀차비시적FISH기술,시령일충유효적선택.
Objective Toestablish a primed in situ labeling(PR1NS) technique which can be more effective in detection of single copy gent.Methods On the basis of traditional PRINS,new reagents and procedures,such as TaqStart antibody,four primers of the sex determining region Y (SRY) gene and TSA TM Biotin System were included in detection of the SRY gene.Meanwhile,fluorescence in situ hybridization(FISH)to detect the SRY gene was used as control.Results Fifty metaphases were scored.PRINS labeling showed signals for the SRY on the Y chromosome at band Yp11.3 in all metaphases.These signals were as distinct as that from resuhs of FISH.Conclusion This improved method is ideal for rapidly localizing single copy genes and small DNA segments.And PRINS is a cost-and time-effective alternative to FISH.