CAJ | 학술논문

研制了磁弹性金黄色葡萄球菌无线传感器,用于检测不同液体介质中的金黄色葡萄球菌.取0.2 mL一定浓度菌液加到含2 mL无菌液体介质的检测玻璃管中,磁弹性传感器共振频率随细菌的生长而改变.通过改变牛肉膏和蛋白胨的浓度,得出传感器在含有2×10~5 cells/mL金黄色葡萄球菌的培养基CM 2-2中共振频率响应最大.结果表明,此传感器可以测定的金黄色葡萄球菌浓度范围在CM 2-2中是3×10~3～2×10~7 cells/mL,在牛奶中是1×10~4～2×10~7 cells/mL,检出限分别是1×10~3 cells/mL和1×10~4 cells/mL.传感器共振频移大小与金黄色葡萄球菌的浓度呈线性相关关系,相关系数在牛奶中是0.98,在培养基中是0.99.
연제료자탄성금황색포도구균무선전감기,용우검측불동액체개질중적금황색포도구균.취0.2 mL일정농도균액가도함2 mL무균액체개질적검측파리관중,자탄성전감기공진빈솔수세균적생장이개변.통과개변우육고화단백동적농도,득출전감기재함유2×10~5 cells/mL금황색포도구균적배양기CM 2-2중공진빈솔향응최대.결과표명,차전감기가이측정적금황색포도구균농도범위재CM 2-2중시3×10~3～2×10~7 cells/mL,재우내중시1×10~4～2×10~7 cells/mL,검출한분별시1×10~3 cells/mL화1×10~4 cells/mL.전감기공진빈이대소여금황색포도구균적농도정선성상관관계,상관계수재우내중시0.98,재배양기중시0.99.
A magnetoelastic Staphylococcus aureus (S.aureus) wireless sensor was fabricated to detect S.aureus in different liquid medium.After 0.2 mL bacteria with proper concentration was taken into the tes ting cuvette containing 2 mL liquid medium, the resonance frequency of magnetoelastic sensor changed with the bacteria growth.Via changing the concentration of beef extract and peptone, sensor had the most frequen cy response in culture medium CM2-2 incubated with 2 × 10~5 cells/mL S.aureus.The results indicated that this sensor can be used to determine S.aureus concentrations of 3 × 10~3 to 2×10~7 cells/mL in culture medium and 10~4 to 10~7 cells/mL in milk, with the detection limits of 10~3 cells/mL and 104 cells/mL, respectively.The sensor frequency shift was correlated to the logarithmic value of the S.aureus concentration, and the correlation coefficient was 0.98 in milk and 0.99 in culture medium.