CAJ | 학술논문

目的:探讨EB病毒在儿童慢性扁桃体炎、腺样体肥大中的流行病学特点及其在儿童腺样体肥大、慢性扁桃体炎疾病发病机制中的作用.方法:采用实时荧光定量PCR技术对52例慢性扁桃体炎、腺样体肥大患儿手术摘除的腺样体、扁桃体组织及血浆标本进行EB病毒定量检测.结果:患有慢性扁桃体炎和(或)腺样体肥大的儿童扁桃体、腺样体组织中EB病毒感染率为51.9%;其中男性患儿EB病毒感染率为50.0%,女性患儿为55.6%,两者差异无统计学意义(P＞0.05).扁桃体组织EB病毒感染率为40.4%,腺样体组织为48.9%,差异无统计学意义(P＞0.05).学龄组(7～14岁)患儿扁桃体和腺样体组织EB病毒感染率为65.5%,明显高于学龄前组(2～6岁)患儿的感染率(34.8%).比较轻、中、重度肥大的腺样体组织中EB病毒-DNA拷贝数发现:重度肥大组EB病毒-DNA拷贝数明显高于其他两组(P＜0.05).52例患儿血浆标本检测发现:EB病毒-DNA拷贝数均在正常范围内(＜1×10~3 copies/ml).结论:腺样体肥大组织与慢性扁桃体炎组织对EB病毒有相同的易感性,男、女性患儿的扁桃体和腺样体组织对EB病毒易感性基本相同,且随着患儿年龄的增长、病程的延长,EB病毒的感染率也会相应增高.腺样体的增生、肥大与EB病毒的感染有一定相关性.
목적:탐토EB병독재인동만성편도체염、선양체비대중적류행병학특점급기재인동선양체비대、만성편도체염질병발병궤제중적작용.방법:채용실시형광정량PCR기술대52례만성편도체염、선양체비대환인수술적제적선양체、편도체조직급혈장표본진행EB병독정량검측.결과:환유만성편도체염화(혹)선양체비대적인동편도체、선양체조직중EB병독감염솔위51.9%;기중남성환인EB병독감염솔위50.0%,녀성환인위55.6%,량자차이무통계학의의(P＞0.05).편도체조직EB병독감염솔위40.4%,선양체조직위48.9%,차이무통계학의의(P＞0.05).학령조(7～14세)환인편도체화선양체조직EB병독감염솔위65.5%,명현고우학령전조(2～6세)환인적감염솔(34.8%).비교경、중、중도비대적선양체조직중EB병독-DNA고패수발현:중도비대조EB병독-DNA고패수명현고우기타량조(P＜0.05).52례환인혈장표본검측발현:EB병독-DNA고패수균재정상범위내(＜1×10~3 copies/ml).결론:선양체비대조직여만성편도체염조직대EB병독유상동적역감성,남、녀성환인적편도체화선양체조직대EB병독역감성기본상동,차수착환인년령적증장、병정적연장,EB병독적감염솔야회상응증고.선양체적증생、비대여EB병독적감염유일정상관성.
Objeetive:To investigate the epidemiology of EBV in adenoidal hypertrophy and chronic tonsillitis and discuss the effection of EBV on the nosogenesis of adenoidal hypertrophy and tonsillitis of children.Method:Fifty-two children with chronic tonsillitis and/or adenoidal hypertrophy had the operations of the tonsillectomy and/or the adenoidectomy.These tissues reseeted and plasma of all cases were detected to find EBV-DNA by RQPCR.Result:The infection rate of EBV in the tissues of adenoidal hypertrophy and tonsillitis of childen was 51.9%.The boys'infection rate of EBV was 50.0%,and the girls'infection rate of EBV was 55.6%,which had not significantly different.The EBV infection rate in the tissues of tonsillitis was 40.4%,The EBV infection rate in the tissues of adenoidal hypertrophy was 48.9%,which had not significant difference.The school age group(7to 14 years old)presented higher infection rate of EBV in the tissues of adenoid and tonsil(65.5%)than the preschool children group(2 to 6 years old)(34.8%).Comparing the copies numbers of BV-DNA in the different degrees of adenoidal hypertrophy,we found that the copies numbers of EBV-DNA in the severe hypertrophy group were higher than the midrange and slight hypertrophy groups(P＜0.05).Meanwhile we detected EBV-DNA in these childrens'blood plasma by RQ-PCR.No blood plasma was detected EBV-DNA copies higher than normal(＜1×10~3 copies/ml).Conclusion:The tissues of adenoidal hypertrophy and tonsillitis had same sensitivity to EBV.There was not significant difference between the infection rates of the boys and girls with adenoidal hypertrophy and/or tonsillitis.With these children growing up and the course of diseases prolonging,the infection rate of EBV increased correspondingly.There was a certain correlation between the hypertrophy of adenoid and EBV.There were NO EBV-DNA fragments in blood plasma of the children with adenoidal hypertrophy and/or tonsillitis.So there were essential different between benign hyperplasy and nasopharyngeal carcinoma.