生物技术
生物技術
생물기술
BIOTECHNOLOGY
2009年
6期
44-45
,共2页
鲁云风%杨柯金%杨建伟%何拉可%刘虹梁
魯雲風%楊柯金%楊建偉%何拉可%劉虹樑
로운풍%양가금%양건위%하랍가%류홍량
细胞融合%蟾蜍红细胞%聚乙二醇%细胞融合率
細胞融閤%蟾蜍紅細胞%聚乙二醇%細胞融閤率
세포융합%섬서홍세포%취을이순%세포융합솔
cell fusion%toad red blood cells%PEG%cell fusion rate
目的:用聚乙二醇法探讨蟾蜍红细胞融合的最佳条件.方法:采用3因素3水平的正交实验法,以蟾蜍红细胞为材料,从聚乙二醇浓度、反应时间、反应温度三方面计算细胞融合率,探讨和比较蟾蜍红细胞融合的最适条件及影响因素.结果:蟾蜍红细胞融合的最佳条件是:选用50%的聚乙二醇,反应温度为37℃,反应时间为15min.
目的:用聚乙二醇法探討蟾蜍紅細胞融閤的最佳條件.方法:採用3因素3水平的正交實驗法,以蟾蜍紅細胞為材料,從聚乙二醇濃度、反應時間、反應溫度三方麵計算細胞融閤率,探討和比較蟾蜍紅細胞融閤的最適條件及影響因素.結果:蟾蜍紅細胞融閤的最佳條件是:選用50%的聚乙二醇,反應溫度為37℃,反應時間為15min.
목적:용취을이순법탐토섬서홍세포융합적최가조건.방법:채용3인소3수평적정교실험법,이섬서홍세포위재료,종취을이순농도、반응시간、반응온도삼방면계산세포융합솔,탐토화비교섬서홍세포융합적최괄조건급영향인소.결과:섬서홍세포융합적최가조건시:선용50%적취을이순,반응온도위37℃,반응시간위15min.
Objective: To study toad red blood cells fusion, the best method is PEG induced fusion, which has the advantages are convenient and get fusion bodies easily.Method: Using three factors and three levels of uniform orthogonal test to find a more effective and easier method of cell fusion. Result: To account the fusion rate of toad red blood cells, three aspects were considered, which are PEG density, reaction time and reaction temperature. Conclusion: The most appropriate condition for cell fusion in the toad red blood cells is 50% PEG, 37 centigrade and 15 minutes, the fusion phenomenon is evident in this condition.
