食品科学
食品科學
식품과학
FOOD SCIENCE
2010年
3期
253-257
,共5页
纳豆芽孢杆菌%米糠%整肠%合生素
納豆芽孢桿菌%米糠%整腸%閤生素
납두아포간균%미강%정장%합생소
Bacillus natto%rice bran%regulating intestinal tract%synbiotics
目的:为深度开发米糠资源,考察纳豆芽孢杆菌发酵的米糠提取物(supematant of Bacillus natto-fermented rice bran,SBRB)的整肠作用.方法:米糠提取物与双歧杆菌的体外共培养试验;灌胃SBRB,检测小鼠肠道正常菌群的生长.结果:体外试验证明纳豆菌在严格厌氧环境小能促进双歧杆菌的生长.加入SBRB与米糠水提液(aqueous extract of rice bran,ABRB)均能显著促进双歧杆菌的生长,提高比率分别为65.2%和17.8%:SBRB经过胃蛋白酶和胰蛋白酶消化后,仍有51.6%的促生率.作为外源因子的SBRB与AERB中含有多种促生成分.动物实验结果表明,灌胃SBRB能够显著促进以厌氧菌为优势菌群的小鼠肠道菌群的生长,抑制好氧菌的增殖,作用效果与微生态制剂培菲康相当;纳豆菌和发酵成分均对小鼠的肠道菌群具有调节作用,两者复合能发挥更强的功能.结论:SBRB具有调整肠道菌群的功能,适于制成合生素制剂.
目的:為深度開髮米糠資源,攷察納豆芽孢桿菌髮酵的米糠提取物(supematant of Bacillus natto-fermented rice bran,SBRB)的整腸作用.方法:米糠提取物與雙歧桿菌的體外共培養試驗;灌胃SBRB,檢測小鼠腸道正常菌群的生長.結果:體外試驗證明納豆菌在嚴格厭氧環境小能促進雙歧桿菌的生長.加入SBRB與米糠水提液(aqueous extract of rice bran,ABRB)均能顯著促進雙歧桿菌的生長,提高比率分彆為65.2%和17.8%:SBRB經過胃蛋白酶和胰蛋白酶消化後,仍有51.6%的促生率.作為外源因子的SBRB與AERB中含有多種促生成分.動物實驗結果錶明,灌胃SBRB能夠顯著促進以厭氧菌為優勢菌群的小鼠腸道菌群的生長,抑製好氧菌的增殖,作用效果與微生態製劑培菲康相噹;納豆菌和髮酵成分均對小鼠的腸道菌群具有調節作用,兩者複閤能髮揮更彊的功能.結論:SBRB具有調整腸道菌群的功能,適于製成閤生素製劑.
목적:위심도개발미강자원,고찰납두아포간균발효적미강제취물(supematant of Bacillus natto-fermented rice bran,SBRB)적정장작용.방법:미강제취물여쌍기간균적체외공배양시험;관위SBRB,검측소서장도정상균군적생장.결과:체외시험증명납두균재엄격염양배경소능촉진쌍기간균적생장.가입SBRB여미강수제액(aqueous extract of rice bran,ABRB)균능현저촉진쌍기간균적생장,제고비솔분별위65.2%화17.8%:SBRB경과위단백매화이단백매소화후,잉유51.6%적촉생솔.작위외원인자적SBRB여AERB중함유다충촉생성분.동물실험결과표명,관위SBRB능구현저촉진이염양균위우세균군적소서장도균군적생장,억제호양균적증식,작용효과여미생태제제배비강상당;납두균화발효성분균대소서적장도균군구유조절작용,량자복합능발휘경강적공능.결론:SBRB구유조정장도균군적공능,괄우제성합생소제제.
Objective:To exploit the resource of rice bran and investigate the regulation role of supematant of Bacillus nattofermented rice bran(SBRB) for intestinal tract system in mice.Methods:The culture supernatant of Bacillus natto in rice bran fermentation medium was co-cultured with Bacillus bifidus.Mice were treated with SBRB via a gavage tube and the quantity of normal flora of intestinal tract in mice was detected.Results:Individual Bacillus natto could not promote the growth of Bacillus bifidus under anaerobic environment in vitro.However,both SBRB and aqueous extract of rice bran(AERB)could promote the growth of Bacillus bifidus by 65.2% and 17.8%,respectively.After enzymatic digestion by pepsin and trypsin,51.6% promotion capacity of SBRB was remained.Similarly,the growth promotion of anaerobic bacteria and growth inhibition of aerobic bacteria were also observed in the intestinal tract in mice treated with SBRB.Individual application of Bacillus natto and its fermentation broth exhibited a regulatory effect on normal flora of intestinal tract in mice.In additions,a synergistic effect of combinatorial application on nollnal flora of intestinal tract in mice was also observed.Conclusion:SBRB has the function for regulating intestinal tract flora and has a promising potential to be used as synbiotic preparation.
