CAJ | 학술논문

背景: 体质量指数(BMI)是研究肥胖的常用表型,它是由多基因决定的复杂性状.目的:验证骨钙素基因Hind Ⅲ多态性是否与体质量指数的变异相关联.方法:征集南昌当地绝绎前女性390例,并测量身高和体质量.用聚合酶链式反应-限制性片段长度多态性(PCR-RFLP)法对所有个体的骨钙素基因Hind Ⅲ位点进仃基因分型.结果与结论:骨钙素HH,Hh和hh基因型频率分别为0．077,0．408和0．515.骨钙素基因型分布符合哈的温格尔平衡(P>0．05).骨钙素基因Hind Ⅲ与体质量指数存在显著的关联(P=0．002),它可以解释大约5 47％体质量指数的变异.HH基因型个体的体质量指数最高[(22．81±0．73)kg/m2],Hh基因型个体的体质量指数居中[(21．50±0．53)kg/m2],而hh基因型个体的体质量指数最低[(20．23±0．63)kg/m2].因此,HH和Hh基因型个体的体质量指数比hh基因型个体的体质量指数分别高大约12．75％和6．28％.文章首次存健康绝经前女性中报道骨钙素基因HindⅢ多态性与体质量指数的变异相关联.
배경: 체질량지수(BMI)시연구비반적상용표형,타시유다기인결정적복잡성상.목적:험증골개소기인Hind Ⅲ다태성시부여체질량지수적변이상관련.방법:정집남창당지절역전녀성390례,병측량신고화체질량.용취합매련식반응-한제성편단장도다태성(PCR-RFLP)법대소유개체적골개소기인Hind Ⅲ위점진정기인분형.결과여결론:골개소HH,Hh화hh기인형빈솔분별위0．077,0．408화0．515.골개소기인형분포부합합적온격이평형(P>0．05).골개소기인Hind Ⅲ여체질량지수존재현저적관련(P=0．002),타가이해석대약5 47％체질량지수적변이.HH기인형개체적체질량지수최고[(22．81±0．73)kg/m2],Hh기인형개체적체질량지수거중[(21．50±0．53)kg/m2],이hh기인형개체적체질량지수최저[(20．23±0．63)kg/m2].인차,HH화Hh기인형개체적체질량지수비hh기인형개체적체질량지수분별고대약12．75％화6．28％.문장수차존건강절경전녀성중보도골개소기인HindⅢ다태성여체질량지수적변이상관련.
BACKGROUND: Body mass index(BMI)is a commonly used phenotype for obesity,which is determined by multiple genetic factors.OBJECTIVE: To investigate whether osteocalcin(also known as bone Gla protein,BGP)Hind Ⅲ genetic polymorphism is associated with BMI variation.METHODS: A total of 390 premenopausal women from a local population of Nanchang City were selected.Body weight and height were measured.All participants were genotyped at the BGP Hind Ⅲ locus using polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP).RESULTS AND CONCLUSION: The BGP genotype frequencies of HH,Hh and hh were 0.077,0.408 and 0.515,respectively.The distribution of BGP Hind Ⅲ genotypes was consistent with Hardy-Weinberg equilibrium(P > 0.05).The BGP Hind Ⅲ were significantly associated with BMI(P=0.002),which could explain about 5.47% of BMI variation.On average,BMI of individuals with HH genotype was the highest[(22.81±0.73)kg/m2],individuals with Hh genotype was intermediate[(21.50±0.53)kg/m2],while individuals with hh genotype was the lowest[(20.23±0.63)kg/m2].Therefore,carriers of HH and Hh genotypes had,respectively,approximately 12.75% and 6.28% higher BMI than carriers of the hh genotype.To our best knowledge,this is the first study reporting the association of BGP Hind Ⅲ genetic polymorphism and BMI in healthy premenopausal women.